高级生物矩阵/HyStem//GS310 2.5毫升试验试剂盒

价格
¥2300.00
货号:GS3102.5mLTrialKit
浏览量:127
品牌:Advanced BioMatrix
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商品描述

ProductDescription

HyStem®HydrogelKits-Theblankslatematrix.

  • Forfirsttimeusers,wehighlyrecommendthe2.5mLor7.5mLkitsize.

HyStem®hydrogelsarexeno-freeandcompletelycustomizable.Recommendedforapplicationsrequiringspecificattachmentfactoroptimizationaswellasnon-adherentcellculture,cellularapplications.Extracellularmatrixproteinscanbemixedintothehydrogelandincorporatednon-covalentlybeforegelation.Alternatively,attachmentpeptideshavinganN-terminalcysteinecanalsobecovalentlylinkedtothematrix.

TheHyStemHydrogelKitsareoptimalforculturingstemcellswhosenaturalenvironmentisrichinhyaluronicacid(HA).HyStemhydrogelscanbecustomizedbyaddingextracellularmatrix(ECM)proteinsorcellattachmentpeptidesintothehydrogeltoprovideattachmentsiteand/ordifferentiationsignals.Theycanalsobevariedbychangingthehydrogelrigiditytomatchthatofthenativecellenvironment.TheHyStemhydrogelsystemisviableforcellcultureinanyformat,fromT-flasksandtissuecultureinsertsto384-wellmicroplates.Features

  • Dimensionality(3-Dencapsulationor2-Dplatingontopofhydrogel)
  • FlexIBLecultureformat(384-through6-wellplates,tissue-cultureinserts,andT-flasks)
  • VariableamountsandtypeofECMproteinincorporated
  • Gelationpropertiesincludinggelationtimeandhydrogelstiffness

Animal-freeSystemHyStemhydrogelsarexeno-freesinceitstwocomponentsarethiol-modifiedhyaluronan(Glycosil®)andathiol-reactivecrosslinker(polyethyleneglycoldiacrylate,Extralink®-Lite).ThehyaluronicacidusedtoproduceHyStemismadebyaproprietarybacterial-fermentationprocessusingbacillussubtilis(Novozymes).Itis100%freeofanimal-derivedrawmaterialsandnoanimal-derivedingredientsareusedinitsproduction.Extralink(polyethyleneglycoldiacrylate)ismadebyaddingacrylategroupstobothendsofapolyethyleneglycol(PEG)polymer.PEGisderivedfrompetroleumandinorganicsourcesandcontainsnoanimalsourcematerials.GelationReconstitutedHyStem-Ccomponentsremainliquidat15to37°Cforseveralhours.Thehydrogelisformedwhenthecrosslinkingagent,Extralink®-Lite(PEGDA)isaddedtoamixtureofGlycosil®(thiol-modifiedhyaluronan.Gelationoccursinabouttwentyminutesafterallthreecomponentsaremixed.NostepsdependonlowtemperaturesorlowpH.Dilutingthecomponentswithphosphate-bufferedsaline(PBS)orcell-culturemediumcanincreasethegelationtime.3DCellRecoveryMatrixForapplicationwherecellrecoveryiscritical,thealternativecrosslinkerPEGSSDAisavailableforusewithallHyStemhydrogelkits.ThiscrosslinkerprovidesthesameadvantagesofferedbyExtralinkwiththeadditionalbenefitofcontainingeasilyreducibleinternalbonds.Thisallowsforfast,easyrecoveryofsinglecellsorclustersfromthehydrogelforapplicationslikeRNAanalysisorflowcytometryinsteadofslowenzymaticmethodsthatcanimpactcellviABIlity.ResearchersareencouragedtocontactustodeterminethecompatibilityofparticularcelltypesorculturesystemswithPEGSSDA.

DirectionsforUse

DownloadtheHyStem®hydrogelkitinstructionsfor:

Catalog#GS3102.5mLTrialKit

Catalog#GS3117.5mLKit

Catalog#GS100412.5mLKit

ProductQ&A

Globularparticleslessthan75kDashouldbeabletofreelydiffusethroughaHyStemhydrogel.

WhenreconstitutedusingDGwater,thepHofeachHyStemcomponentwillbeapproximately7.4-7.6.

Oneyearfromthedateofreceipt,ifstoredproperly.

Anysterile,deionized,degassedwatercanbesubstitutedforreconstitution.However,inordertoensureaccurateandpredictabledissolutionandgelationtimes,ourDGWaterishighlyrecommended,asitisdegassed,blanketedinargon,andhasundergonevalidationtestingwitheachHyStemcomponent.

Gelin-Sprovidescellularattachmentsiteswhenincorporatedinthehydrogel.Gelin-Sisthiol-modified,denaturedcollagenI,derivedfromeitherbovineorporcinesources.Gelin-SisincludedinallHyStem-CandHyStem-HPkits.

Gelin-Shasbeenthiol-modifiedinthesamemannerasthehyaluronaninGlycosil(orHeprasil),sothatitcovalentlycrosslinkswiththeExtralinkintheHyStemhydrogels.

Yes.Peptidesthatcontainacysteineresiduecanbeused.Thecysteineresiduemustbepresentforthepeptidetobecovalentlybondedtothehydrogelsubstrate.

Yes.ECMproteins,suchaslaminin,collagen,fibronectin,orvitronectincanbenon-covalentlyincorporatedintothehydrogelpriortocrosslinking.

HyStemhydrogelsandspongesdifferinhydrationandhomogeneity.HyStemspongesaretypicallypolymerizedhydrogelsthataresubsequentlyfreeze-dried.Theresultingspongeisafibrous,meshnetworkwithporesandnichesthatenablecellstoinfiltrateandadhere.AtrueHyStemhydrogelisanencapsulatingliquidthatpolymerizesaroundsUSPendedcellsinculture.

No.ThecomplianceofthehydrogelsissetbytheamountofExtralinkcrosslinkeradded,theconcentrationofGlycosil(orHeprasil)andGelin-Sused,andtheratioofGlycosil(orHeprasil)toGelin-S.Oncethischemicalstructureofthehydrogelisfixed,itisnotalteredbyprolongedexposuretocellculturemedium.

HyStemspongescanbeterminallysterilizedbyE-beam.HyStemhydrogelshavenotyetbeenvalidatedforusewithE-beamsterilizationmethods.HyStemhydrogelsarenotterminallysterilizedbygammairrADIation.

Gelationtimeisaffectedbymultipleaspectsofthegel’scomposition.Onewaytochangethegelationtimeofahydrogelistovarytheamountofcrosslinkerused.GelswithaloweramountofExtralinkcrosslinkerwillhavealongergelationtimethanthosewithahigheramountofcrosslinker.Changingtheamountofcrosslinkerwillproduceslightchangesingelationtime.GelationtimecanbedramaticallychangedbyvaryingtheGlycosil(orHeprasil)andGelin-Sconcentrations.ConcentratedsolutionsofGlycosil(orHeprasil)andGelin-Swillcreateasolutionwithamuchshortergelationtime.ThiscaneasilybedonebyreconstitutingthecomponentsinasmallervolumeofDGWater.Alternatively,dilutingthesecomponentsinlargervolumesofDGWaterwilldramaticallyincreasethetotaltimetoformthehydrogel.

HyStemHydrogelsarevirtuallytransparentandshouldnotinterferewithmicroscopy.

HyStemhydrogelsmaygeneratemildinflammationaspartofthebody’snaturalhealingprocessinresponsetoinjury.HyStemhydrogelsdonottriggerimmuneresponsewhenusedinvivo.(Theseproductsarenotforhumanuse)

HyStemisdegradedinvivobymatrixmetalloproteinases(Collagenases)andhyaluronidases.

Trypsin,Dipase,collagenase,andhyaluronidasehavebeenusedtohelpdetachcellsfromthesurfaceorfromwithinHyStemhydrogels.

Ingeneral,theporesizeforHyStem-CandHyStem-HPhydrogelsis~17nm.

ProductApplications

Clickonthetitleofthedesiredprotocoltolearnmore:

2DCellGrowthonHyStemHydrogels

HyStem3DCellEncapsulationforCellDeliveryApplicationsGuide

HyStem3DCellEncapsulationinhydrogelsusing96-wellplates

HyStem3DCellEncapsulationinhydrogelsusingTCInserts

EnzymeDigestionofHyStemHydrogelsforRecoveryofEncapsulatedCells

FluorescentLabelingofHyStemHydrogels

CellRecoveryfromSurfaceofHyStemHydrogels

HyStemECMIncorporation

HyStemGelationTimeVariation

HyStemStiffnessVariationProtocolfor7.5mLkit

HyStemStiffnessVariationProtocolfor12.5mLkit

ProductReferences

ReferencesforHyStem®:

Gaetani,R.,etal.(2015)EpicardialapplicationofcardiacProgenitorcellsina3D-printedgelatin/hyaluronicacidpatchpreservescardiacfunctionaftermyocardialinfarction.Biomaterials61:339-348.PMID:17335875.Prestwich,G.D.,etal.(2007)3-Dcultureinsyntheticextracellularmatrices:newtissuemodelsfordrugtoxicologyandcancerdrugdiscovery.AdvEnzymeRegul47:196-207.PMID:17335875.Shu,X.Z.,etal.(2006)Synthesisandevaluationofinjectable,insitucrosslinkablesyntheticextracellularmatricesfortissueengineering.JBiomedMaterResA79:901-912.PMID:16941590.Shu,X.Z.,etal.(2003)Disulfide-crosslinkedhyaluronan-gelatinhydrogelfilms:acovalentmimicoftheextracellularmatrixforinvitrocellgrowth.Biomaterials24:3825-3834.PMID:12818555.

S.Cai,etal.(2005)Injectableglycosaminoglycanhydrogelsforcontrolledreleaseofhumanbasicfibroblastgrowthfactor.Biomaterials,26,6054-6067.D.B.Pike,etal.(2006)Heparin-regulatedreleaseofgrowthfactorsinvitroandangiogenicresponseinvivotoimplantedhyaluronanhydrogelscontainingVEGFandbFGF.Biomaterials,27,5242–5251.G.D.Prestwich,etal.(2007)3-DCultureinSyntheticExtracellularMatrices:NewTissueModelsforDrugToxicologyandCancerDrugDiscovery.invited,Adv.Enz.Res.,inpress(2007).X.Z.Shu,etal,(2006)SynthesisandEvaluationofInjectable,InSituCrosslinkableSyntheticExtracellularMatrices(sECMs)forTissueEngineering.J.BiomedMater.Res.A,79A(4),901-912.

Shu,X.Z.,etal.(2004)Insitucrosslinkablehyaluronanhydrogelsfortissueengineering.Biomaterials25:1339-1348.PMID:14643608.Mehra,T.D.,etal.(2006)Molecularstentingwithacrosslinkedhyaluronanderivativeinhibitscollagengelcontraction.JInvestDermatol126:2202-2209.PMID:16741511.Shu,X.Z.,etal.(2004)AttachmentandspreadingoffibroblastsonanRGDpeptide-modifiedinjectablehyaluronanhydrogel.JBiomedMaterResA68:365-375.PMID:14704979.Ghosh,K.,etal.(2007)CelladaptationtoaphysiologicallyrelevantECMmimicwithdifferentviscoelasticproperties.Biomaterials28:671-679.PMID:17049594.

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ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

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