高级生物基质/纤维连接蛋白//5050-1MG

价格
¥2400.00
货号:5050-1MG
浏览量:127
品牌:Advanced BioMatrix
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商品描述

Product Description

Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds. Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs).
Fibronectin is ideal for coating of surfaces and is typically used at a concentration of 1-5 ug/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice.
Parameter, Testing, and MethodFibronectin#5050
Quantity1.0 mg
Volume2.0 mL
Concentration0.5 mg/mL
Purity - SDS PAGE Electrophoresis>95%
Formulation20 mM Tris-HCL, pH 7.0 containing 0.45 M NaCl and 12% Glycerol
FormSolution
SourceHuman, Plasma
Storage Temperature-20°C
Shelf LifeMinimum of 6 months from date of receipt
Sterilization MethodFiltration
Cell Attachment AssayPasses
Sterility - USP modifiedNo growth
SafetySource material found negative for infectious agents

Directions for Use

Download the full PDF versionor continue reading below:

Coating Procedures:

Use these recommendations as guidelines todetermine the optimal coating conditions for yourculture system.

  1. Thaw Fibronectin and dilute to desiredconcentration using serum-free medium orPBS (Ca++, Mg++ free). The final solutionshould be sufficiently dilute so that thevolume added covers the surface evenly.
  2. Add appropriate amount of dilutedmaterial to culture surface.
  3. Incubate at room temperature forapproximately 1 hour.
  4. Aspirate remaining material.
  5. Rinse plates carefully with dH2O– avoidscratching bottom surface of plates.
  6. Plates are ready for use. They may alsobe stored at 2-8°C damp or air dried ifsterility is maintained.

Product Q & A

440 kDa. The protein is a dimer of two 220 kDa identical subunits. It shows up as 220 kDa on a reduced gel, and 440 kDa on a non-reduced gel.

Product References

References for Fibronectin:

Alhussein, Ghada, et al. "A spatiotemporal characterization method for the dynamic cytoskeleton."Cytoskeleton73.5 (2016): 221-232.

Pezzoli, Daniele, et al. "Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds."Biomaterials180 (2018): 130-142.

Wiley, Luke A., et al. "Generation of xeno‐free, cGMP‐compliant patient‐specific iPSCs from skin biopsy."Current protocols in stem cell biology42.1 (2017): 4A-12.

Wiley, Luke A., et al. "Using patient-specific induced pluripotent stem cells and wild-type mice to develop a gene augmentation-based strategy to treat CLN3-associated retinal degeneration."Human gene therapy27.10 (2016): 835-846.

Worthington, Kristan S., et al. "Two-photon polymerization for production of human iPSC-derived retinal cell grafts."Acta biomaterialia55 (2017): 385-395.

Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes."Human Cell Culture Protocols. Humana Press, 2012. 15-29.

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle."Journal of tissue engineering and regenerative medicine12.2 (2018): e918-e936.

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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