Journal ArticleCation-exchange displacement chromatography for the purification of recombinant protein therapeutics from variantsBarnthouse KTrompeter WJones R et al.See moreJournal of Biotechnology (1998) 66(2-3) 125-136DOI: 10.1016/S0168-1656(98)00131-X44CitationsCitations of this article23ReadersMendeley users who have this article in their library.Add to library Get full textAbstract Keywords (8)AbstractRemoval of low level impurities that are closely related to the bioproduct is a commonly encountered challenge in the purification of biopharmaceuticals. These separations are typically carried out by using shallow linear salt gradients at relatively low column loadings, significantly limiting the throughput of the purification process. In this manuscript we examine the utility of displacement chromatography for the purification of recombinant human brain-derived neurotrophic factor, rHuBDNF. The utility of displacement chromatography is compared to gradient elution for the removal of variants of the rHuBDNF. The results demonstrate that displacement chromatography is capable of achieving high yields and purity at high column loadings. Displacements developed on 20 μm and 50 μm cation-exchange resins are shown to provide 8-fold and 4.5-fold increases in production rates, respectively, when compared to an existing linear gradient elution operation. These results demonstrate the efficacy of displacement chromatography for the purification of therapeutic proteins from complex feed streams. Copyright (C) 1998 Elsevier Science B.V.Author supplied keywordsComparisonComplex mixtureDisplacement chromatographyProductivityProtamine sulfateProtein purificationVariantrHuBDNFRegister to see more suggestionsMendeley helps you to discover research relevant for your work.Already have an account? Sign inSign up for freeCiteCITATION STYLEAPABarnthouse, K. A., Trompeter, W., Jones, R., Prasad Inampudi, Rupp, R., Cramer, S. M. (1998). Cation-exchange displacement chromatography for the purification of recombinant protein therapeutics from variants. Journal of Biotechnology, 66(2–3), 125–136. https://doi.org/10.1016/S0168-1656(98)00131-XReaders SeniorityPhD / Post grad / Masters / Doc 750%Researcher 536%Professor / Associate Prof. 214%Readers DisciplineAgricultural and Biological Sciences 853%Chemical Engineering 320%Medicine and Dentistry 320%Pharmacology, Toxicology and Pharmaceut... 17%

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