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蚂蚁淘在线 / 品牌中心 / Signosis / Signosis/大鼠P450 cDNA平板阵列/AP-1181/1 Ea

Signosis/大鼠P450 cDNA平板阵列/AP-1181/1 Ea

价格
￥10480.00
货号：AP-1181
浏览量：127
品牌：Signosis
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商品描述

Description:

ThecytochromeP450familyofenzymesplaycentralrolesintheoxidativemetabolismofawiderangeofxenobiotics,includingchemicals,sterols,fattyacidsandanticancerdrugs. HumanCYPgenesencodefifty-sevenP450proteinswithnumeroushomologvariantsindifferenttissues.Theyarehighlydifferentiallyexpressedinresponsetoavarietyofenvironmentalchemicalsandpharmaceuticals. 

ApplicableGrid:

ListofApplicableGenes

 123456789101112
ACYP1a1Cyp2d22CYP2s1CYP1a1Cyp2d22CYP2s1CYP1a1Cyp2d22CYP2s1CYP1a1Cyp2d22CYP2s1
BCyp1a2Cyp2d26Cyp3a13Cyp1a2Cyp2d26Cyp3a13Cyp1a2Cyp2d26Cyp3a13Cyp1a2Cyp2d26Cyp3a13
CCyp1b1Cyp2d40Cyp3a16Cyp1b1Cyp2d40Cyp3a16Cyp1b1Cyp2d40Cyp3a16Cyp1b1Cyp2d40Cyp3a16
DCyp2a12Cyp2d9Cyp3a41aCyp2a12Cyp2d9Cyp3a41aCyp2a12Cyp2d9Cyp3a41aCyp2a12Cyp2d9Cyp3a41a
ECYP2a22CYP2d9Cyp4a32CYP2a22CYP2d9Cyp4a32CYP2a22CYP2d9Cyp4a32CYP2a22CYP2d9Cyp4a32
FCyp2b10Cyp2e1Cyp4b1Cyp2b10Cyp2e1Cyp4b1Cyp2b10Cyp2e1Cyp4b1Cyp2b10Cyp2e1Cyp4b1
GCyp2b13Cyp2f218S-rRNACyp2b13Cyp2f218S-rRNACyp2b13Cyp2f218S-rRNACyp2b13Cyp2f218S-rRNA
HCyp2d10Cyp2j6BlankCyp2d10Cyp2j6BlankCyp2d10Cyp2j6BlankCyp2d10Cyp2j6Blank

Principle:

Signosis’proprietaryCDNAplatearrayisaplate-basedhybridizationprofilinganalysisformonitoringtheexpressionofdozensofgenesthroughreversetranscriptionofmRNAintocDNA.Likearrayanalyses,totalRNAisfirstreversetranscribedintocDNAinthepresenceofbiotin-dUTPintheassay.Targetedgenesarethenspecificallycapturedontoindividualwellsonaplate,insteadofmembranes,throughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity.

Data:



AnalysisofexpressionofP450geneswithP450cDNAPlateArray. 2ugofRNAsfromratbrainwassubjectedtoP450cDNAplateassay.ThecDNAplatearrayweredetectedwithHRPbyaluminometerplatereader.

Signosis基因功能分析，靶标发现和确认，分析开发以及化合物筛选通常需要基于细胞的分析。经过工程改造以通过转基因整合到宿主基因组中表达目的基因的稳定细胞系提供了进行此类分析的有效方法。然而，由于建立这种细胞系的过程繁琐，开发过程耗时，劳动，试剂和材料的昂贵以及单个克隆的转染后验证，是否值得仍然值得商de在单个实验室中开发这种稳定细胞系的方法。  为了促进研究，Signosis现在提供了一系列基因表达或荧光素酶报告基因就绪的稳定细胞系。 萤光素酶报道基因稳定细胞系EGFR稳定表达细胞系HER2（ERBB2）稳定表达的细胞系pLenti-MSCV-EGFR-GFP载体pCMV-EGFR载体pCMV-EGFR-IRES-GFP载体pMSCV-EGFR-GFP载体EGFR突变荧光素酶表达载体GAL4-UAS-luc报告基因稳定细胞系NR LBD驱动的GAL4 Reporter稳定细胞系亲代细胞系