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蚂蚁淘在线 / 品牌中心 / Signosis / Signosis/人P450 cDNA板阵列/AP-0202/1 Ea

Signosis/人P450 cDNA板阵列/AP-0202/1 Ea

价格
￥10480.00
货号：AP-0202
浏览量：127
品牌：Signosis
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Description:

ThecytochromeP450familyofenzymesplaycentralrolesintheoxidativemetabolismofawiderangeofxenobiotics,includingchemicals,sterols,fattyacidsandanticancerdrugs. HumanCYPgenesencodefifty-sevenP450proteinswithnumeroushomologvariantsindifferenttissues.Theyarehighlydifferentiallyexpressedinresponsetoavarietyofenvironmentalchemicalsandpharmaceuticals. 

ApplicableGrid:

ListofApplicableGenes

 123456789101112
ACYP1A1CYP2E1CYP11B1CYP1A1CYP2E1CYP11B1CYP1A1CYP2E1CYP11B1CYP1A1CYP2E1CYP11B1
BCYP1A2CYP3A4CYP17A1CYP1A2CYP3A4CYP17A1CYP1A2CYP3A4CYP17A1CYP1A2CYP3A4CYP17A1
CCYP1B1CYP3A5/A7CYP24A1CYP1B1CYP3A5/A7CYP24A1CYP1B1CYP3A5/A7CYP24A1CYP1B1CYP3A5/A7CYP24A1
DBeta-actinGAPDHCYP26A1Beta-actinGAPDHCYP26A1Beta-actinGAPDHCYP26A1Beta-actinGAPDHCYP26A1
ECYP2A6CYP4A11CYP26B1CYP2A6CYP4A11CYP26B1CYP2A6CYP4A11CYP26B1CYP2A6CYP4A11CYP26B1
FCYP2B6CYP4B1CYP27A1CYP2B6CYP4B1CYP27A1CYP2B6CYP4B1CYP27A1CYP2B6CYP4B1CYP27A1
GCYP2C19 CYP4F2/F3/F8CYP39A1CYP2C19 CYP4F2/F3/F8CYP39A1CYP2C19 CYP4F2/F3/F8CYP39A1CYP2C19 CYP4F2/F3/F8CYP39A1
HCYP2D6CYP11A1CYP46A1CYP2D6CYP11A1CYP46A1CYP2D6CYP11A1CYP46A1CYP2D6CYP11A1CYP46A1

Principle:

Signosis’proprietaryCDNAplatearrayisaplate-basedhybridizationprofilinganalysisformonitoringtheexpressionofdozensofgenesthroughreversetranscriptionofmRNAintocDNA.Likearrayanalyses,totalRNAisfirstreversetranscribedintocDNAinthepresenceofbiotin-dUTPintheassay.Targetedgenesarethenspecificallycapturedontoindividualwellsonaplate,insteadofmembranes,throughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity.

Data:



AnalysisofexpressionofP450geneswithP450cDNAPlateArray.2ugofRNAsfromMCF7,HL-60,HeLaand293celllinesweresubjectedtoP450cDNAplateassay.ThecDNAplatearrayweredetectedwithHRPbyaluminometerplatereader.

Signosis基因功能分析，靶标发现和确认，分析开发以及化合物筛选通常需要基于细胞的分析。经过工程改造以通过转基因整合到宿主基因组中表达目的基因的稳定细胞系提供了进行此类分析的有效方法。然而，由于建立这种细胞系的过程繁琐，开发过程耗时，劳动，试剂和材料的昂贵以及单个克隆的转染后验证，是否值得仍然值得商de在单个实验室中开发这种稳定细胞系的方法。  为了促进研究，Signosis现在提供了一系列基因表达或荧光素酶报告基因就绪的稳定细胞系。 萤光素酶报道基因稳定细胞系EGFR稳定表达细胞系HER2（ERBB2）稳定表达的细胞系pLenti-MSCV-EGFR-GFP载体pCMV-EGFR载体pCMV-EGFR-IRES-GFP载体pMSCV-EGFR-GFP载体EGFR突变荧光素酶表达载体GAL4-UAS-luc报告基因稳定细胞系NR LBD驱动的GAL4 Reporter稳定细胞系亲代细胞系