Anti-Urease Antibody Properties Description Anti-UREASE (Jack Bean) (RABBIT) Antibody - 100-4182 Host Rabbit Target Species Jack Bean Known Cross Reactivity Jack Bean (Canavalia ensiformis) Clonality Polyclonal Application ELISA : 1:5,000 - 1:20,000 Western Blot : 1:500 - 1:5,000 ImmunoPrecipitation: 1:100 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format Antiserum Label Unconjugated Concentration 90 mg/mL by Refractometry Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 2.0 mL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName UREA_CANEN Relevant Links UniProtKB - P07374NCBI - AAA83831.1

Anti-Urease Antibody Description

Background Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein.Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research. Synonyms rabbit anti-Anti-Urease Antibody, Urease, Urea amidohydrolase Immunogen Urease [Jack Bean] Immunogen Type Native Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note Anti-Urease Antibody has been assayed against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Urease Antibody. Purity/Specifity Anti-Urease (Jack Bean) (Rabbit) Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Urease [Jack Bean].  Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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