AHSP Antibody Properties Description Anti-AHSP (RABBIT) Antibody - 100-401-E79 Host Rabbit Target Species Human Known Cross Reactivity human, mouse Clonality Polyclonal Application ELISA : 1:2,000 - 1:10,000 Western Blot : 1:500-1:1000 Immunohistochemistry: 1:8000 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format Antiserum Label Unconjugated Concentration 115 mg/mL by Refractometry Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 100 µL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName AHSP Relevant Links UniProtKB - Q9NZD4NCBI - NP_057717.1GeneID - 51327

AHSP Antibody Description

Background AHSP Antibody detects Alpha hemoglobin stabilizing protein (AHSP). AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. AHSP binds free a-globin to promote its folding and inhibit its ability to produce damaging reactive oxygen species. Reduced AHSP levels correlate with increased severity of b-thalassemia in some human cohorts. Synonyms rabbit anti-AHSP antibody, Alpha-hemoglobin-stabilizing protein (AHSP), Erythroid differentiation-related factor, Erythroid-associated factor, EDRF, ERAF Immunogen Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein. Immunogen Type Recombinant Protein Storage Condition Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-AHSP Antibody is suitable for western blotting, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by the end user. Purity/Specifity Anti-AHSP Antibody is directed against the human AHSP protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross reactivity with human and mouse. Cross-reactivity with AHSP from other sources have not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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