Description:

BacPAK6Sec+linearizedbaculovirusDNAisthelatestbaculovirusexpressionsystemtocomeoutoftheOETlabs.BytakingtheoriginalBacPAK6systemandgeneticallymodifyingit,BacPAK6Sec+isabletoincreasetheyieldofrecombinantproteinandtheeaseofharvesting.

BacPAK6Sec+containsadeletionofthechitinasegene(chiA)fromtheoriginalBacPAK6viralbackbone.

Chitinasehasbeenshownbybothconfocalandelectronmicroscopytolocatetotheendoplasmicreticulum,whereitisdenselypackedinaparacrystallinearray,whichseverelyinhibitsthefunctionandefficacyofthesecretorypathway.BydeletingthechiA,secretionoftargetproteinsintothecytoplasmisincreased,greatlyenhancingtheyieldofharvestablerecombinantprotein.

TheconceptofdeletingthechiAgenecameafterthesuccessoftheflashBAC™platformthatutilizesthesamegeneticdeletionforincreasedrecombinantproteinyield.TofindoutmoreabouttheflashBAC™system,clickhere.

BacPAK6linearDNAistheoriginalconvenient,highlyefficientreagentforgeneratingrecombinantbaculovirusexpressionvectors. Itcomprisesamodified Autographacalifornica nucleopolyhedrovirus(AcMNPV)genomewith lacZ insertedinplaceofthenativepolyhedringenecodingregionforconvenientblue/whiteselection.

DigestionofBacPAK6DNAatthreesiteswithBsu36Iremoves lacZ andpartofORF1629,whichisanessentialAcMNPVgene. ThelinearvirusDNAisunabletoinitiateareplicationcycleininsectcells.
Co-transfectionofinsectcellswithlinearBacPAK6DNAandacompatIBLetransfervectorcontainingthecompleteORF1629andaforeigngeneofinterestresultsinrecombinationandrestorationofthecircular,infectiousvirusgenome. Over95%ofthevirusderivedfromtheco-transfectioncomprisesrecombinantvirus. Usually,asingleplaqueassaytitrationincubatedwithX-galandcounterstainedwithneutralredissufficienttodifferentiateparentalbluefromrecombinantwhite(colourless)plaques. ThesecanbereADIlyisolatedandamplifiedtoworkingstocksofpurifiedrecombinantvirusinafewdays.

TheBacPAK6Sec+reagentfromOETLtdcomprisestransfection-ready,linearized(Bsu36I-digested)virusDNA. Itisavailablein5,24and96reactionpacks. Forbulkpurchases,pleasecontactusoninfo@oetltd.com.

WeofferapromotionaldealonallBacPAK6productstoincludeafree3reactionflashBAC™kittohelpyoudecideifmakingtheswitchistherightchoiceforyourlab.

Oxford Expression Technologies是杆状病毒蛋白表达系统的专家。我们所有的产品都经过优化，以确保快速，直接的重组病毒生产和蛋白质表达。我们的在线商店提供了您可能需要的一切，从革命性的 Flash BAC™杆状病毒表达载体和杆状病毒qPCR滴定试剂盒，到转染试剂和转移质粒。