卢西根/万事达放大器™ 含甜菜碱/ME81210/10 mL的10X PCR增强子

价格
¥5500.00
货号:ME81210
浏览量:127
品牌:Lucigen
服务
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商品描述

OptimizePCRreactionsthatusedifferentenzymesthantheFailSafe™System(e.g.Taq,Pfu,andTth).

  • Epicentre ProductFast,simpleoptimization
  • ExtremelyhighsensitivityandspecificityusingthePCREnhancerTechnology
  • MasterAmpPCRPreMixescontainallcomponentsneededforsuccessfulPCR
  • GeneratesPCRproductssuitableforbothTAcloningandblunt-endcloning
  • UsefortheoptimizationofPCRreactionsusingavarietyofdifferentenzymesincludingTaq,Pfu,Tth,Tflpolymerases
Applications
  • SimplifiedPCRoptimizationwithaThermostableDNApolymeraseofchoice.
  • PCRofdifficultorhigh-GCtemplates.
  • PCRamplificationsupto20kb.
  • MultiplexPCR.

Forrapid,dependableoptimizationofPCR,weencourageyoutotrytheFailSafe™PCRSystemtoobtainthebestPCRresultswithanytemplateupto~20kb.However,ifyouprefertouseanotherenzyme,EpicentrealsohastwoMasterAmp™PCROptimizationKitsthatwillhelpyoutoobtainthebestpossIBLePCRresultswiththermostableenzymesfrequentlyusedinPCR,suchasTaq,Pfu,Tth,TflDNApolymerases.TheseMasterAmpPCROptimizationKitscontainaseriesof12MasterAmpPCR2XPreMixesthathavebeenmeticulouslytestedandrepresentacompleterangeofPCRconditionsfrequentlyencountered.EachMasterAmp2XPreMixcontainsallcomponentsneededforPCRexceptfortheprimers,thetemplate,andthePCRenzyme.

First,usetheMasterAmp™PCROptimizationKit(Cat.No.MO7201)toperformPCRwithyourtemplate,primers,andeachofthe12MasterAmpPreMixes.SelectthePreMixthatgivesoptimalresults.Then,usethesameMasterAmpPCRPreMixtoobtainreliable,consistentPCRusngthesameDNAtarget/primerset.

STEP1.FindtheoptimalMasterAmpPCRPreMix.DoPCRwithyourtemplate,primersandPCRenzymeinthe12PreMixes.

A.A75%-GC-richApoEgeneregionwasamplifiedintotalhumangenomicDNAusingPreMixesA-L.MasterAmpPCRPreMixKproducedoptimalresultsinStep1.

STEP2.UsethesameMasterAmpPCRPreMixtoobtainreliable,consistentPCRofthesamesequence.

B.SubsequentPCRoftheApoEgenewiththesameprimerpairandMasterAmp™PreMixKgaveconsistentresults.SubsequentPCRresultsareconsistentandreliableusingtheoptimalPreMixinStep1.

MasterAmpPCROptimizationKits;MasterAmpTaqDNAPolymerase;MasterAmpTflDNAPolymerase.Purchaseofthisproductincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser'sowninternalresearch.Nootherpatentrights(suchas5´NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.

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美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。