
- Lucigen特约代理_Lucigen
- Lucigen公司,LGC代理商,Lucigen经销商,LGC厂家直采,Lucigen官网...
- 请问大家中国北方工业公司(北京)笔试成绩出来 ...
- Lucigen 特约代理_文章
- epicentre中国代理,Lucigen中国代理,蓝景科信价格厂家:蓝景科信(...
- Frontiers | Limited Correlation of Shotgun Metagenomics...
- EconoTaq PLUS 2X Master Mix Lucigen
- 什么是lucigen产品 总代理中北林格为您略作讲解
- lucigen酶制剂产品分类及货号详情
- EconoTaq PLUS 2X Master Mix Lucigen
- 研究 STING 的 popular 工具
- 大肠杆菌电转化感受态细胞制备及转化方法 实验方法

OptimizePCRreactionsthatusedifferentenzymesthantheFailSafe™System(e.g.Taq,Pfu,andTth).
Fast,simpleoptimization
- ExtremelyhighsensitivityandspecificityusingthePCREnhancerTechnology
- MasterAmpPCRPreMixescontainallcomponentsneededforsuccessfulPCR
- GeneratesPCRproductssuitableforbothTAcloningandblunt-endcloning
- UsefortheoptimizationofPCRreactionsusingavarietyofdifferentenzymesincludingTaq,Pfu,Tth,Tflpolymerases
Applications
Forrapid,dependableoptimizationofPCR,weencourageyoutotrytheFailSafe™PCRSystemtoobtainthebestPCRresultswithanytemplateupto~20kb.However,ifyouprefertouseanotherenzyme,EpicentrealsohastwoMasterAmp™PCROptimizationKitsthatwillhelpyoutoobtainthebestpossIBLePCRresultswiththermostableenzymesfrequentlyusedinPCR,suchasTaq,Pfu,Tth,TflDNApolymerases.TheseMasterAmpPCROptimizationKitscontainaseriesof12MasterAmpPCR2XPreMixesthathavebeenmeticulouslytestedandrepresentacompleterangeofPCRconditionsfrequentlyencountered.EachMasterAmp2XPreMixcontainsallcomponentsneededforPCRexceptfortheprimers,thetemplate,andthePCRenzyme. First,usetheMasterAmp™PCROptimizationKit(Cat.No.MO7201)toperformPCRwithyourtemplate,primers,andeachofthe12MasterAmpPreMixes.SelectthePreMixthatgivesoptimalresults.Then,usethesameMasterAmpPCRPreMixtoobtainreliable,consistentPCRusngthesameDNAtarget/primerset. | ![]() | STEP1.FindtheoptimalMasterAmpPCRPreMix.DoPCRwithyourtemplate,primersandPCRenzymeinthe12PreMixes. |
A.A75%-GC-richApoEgeneregionwasamplifiedintotalhumangenomicDNAusingPreMixesA-L.MasterAmpPCRPreMixKproducedoptimalresultsinStep1. | ||
![]() | STEP2.UsethesameMasterAmpPCRPreMixtoobtainreliable,consistentPCRofthesamesequence. | |
B.SubsequentPCRoftheApoEgenewiththesameprimerpairandMasterAmp™PreMixKgaveconsistentresults.SubsequentPCRresultsareconsistentandreliableusingtheoptimalPreMixinStep1. |
MasterAmpPCROptimizationKits;MasterAmpTaqDNAPolymerase;MasterAmpTflDNAPolymerase.Purchaseofthisproductincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser'sowninternalresearch.Nootherpatentrights(suchas5´NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.
Ep