TheTransforMax™EC100D™pir⁺ElectrocompetentE.coliandTransforMax™EC100D™pir-116ElectrocompetentE.colieachconstitutivelyexpresstheπprotein(thepirgeneproduct)forreplicationofplasmidscontainingtheR6Kγoriginofreplication(R6Kγori).ThecellsarederivedfromEpicentre'sTransforMax™EC100™ElectrocompetentE.colibyP1phagetransductionwithastraincontainingthepir⁺orpir-116genelinkedtoadihydrofolatereductase(DHFR)Marker.

TheTransforMaxEC100Dpir⁺cellswillmaintainR6Kγoricontainingplasmidsatapproximately15copiespercell,¹forcloningofpotentiallytoxicorunstableDNAsequences.TheTransforMaxEC100Dpir-116cellsareforhigh-copypropagationofupto250rescueplasmidcopiespercell.¹

Benefits

• AcceptslargeclonesforunbiasedpropagationandstABIlityoflargerescueclones.
• lacZΔM15forblue/whitescreeningofrecombinants.
• Restrictionminus[mcrA,Δ(mrr-hsdRMS-mcrBC)]enablesefficientcloningofmethylatedDNA.
• Endonucleaseminus(endA1)toensurehighyieldsofDNA.
• Recombinationminus(recA1)forgreaterstabilityoflargeclonedinserts.

Genotypes

TransforMaxEC100Dpir⁺ElectrocompetentE.coli:
F^-mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(Str^R)nupGpir⁺(DHFR)

TransforMaxEC100Dpir-116ElectrocompetentE.coli:
F^-mcrAΔ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(Str^R)nupGpir-116(DHFR)

TransformationEfficiency
Greaterthan5×10⁹colonyformingunits(cfu)/µgwithpR6KancontrolDNA.

References

1. Metcalf,W.W.etal.(1994)Gene138,1.

ORDERINFORMATION

Eachproductcontains5tubeseachcontaining100µLofelectrocompetentcells,whichisenoughcellsfor10electroporations.AcontrolvectorcontainingandR6Kγoriisalsoincludedwitheachproduct. 美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。