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- Bestvalueforroutineproteinexpression...availableasElectrocompetentandChemicallyCompetentCells.
Lucigen’sE.cloniEXPRESSBL21(DE3)ElectrocompetentCellsarethefirsttoofferhighefficiencycloningandhighlevelproteinexpressioninthesamecell.Cloningefficienciesareincreased25-1,000foldrelativetocompetitors’preparationsofBL21cells(Figure1),whichisessentialforconstructionofcomplexexpressionlibraries.TheunprecedentedtransformationefficiencyofE.cloniEXPRESSElectrocompetentCells(>5×109cfu/µg)eliminatestheneedforplasmidtransferfromthecloningstraintotheexpressionstrain,savingdaysofworkinatypicalcloningandexpressionexperiment(Figure2).
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Figure1.TransformationefficiencycomparisonofcommerciallyavailableBL21(DE3)competentcells.
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Figure2.Comparisonofcloningandproteinexpressionprocedures. |
E.cloniEXPRESSBL21(DE3)strainscontainthephageT7RNApolymerasegenelinkedtotheIPTG-inducIBLepromoter,forusewithanyexpressionplasmidcontainingtheT7promoter.
NOTE:Forbestresultsinexpressingproteinsthatmaybetoxic,werecommendusingOverExpressCells.E.cloniEXPRESScellsarealsoavailableaschemicallycompetentcells.Withatransformationefficiencyof>1×107cfu/µgandveryeconomicalprices,EXPRESSChemicallyCompetentCellsoffersuperiorvalueforeverydayproteinexpressionwork.
ORDERINFORMATION
EachE.cloniEXPRESSKitcontains:theindicatedE.cloniEXPRESSElectrocompetentorChemicallyCompetentCellsinDUOpackaging(2transformationspertube),ExpressionRecoveryMedium(lactoseminus),pUC19PositiveControlPlasmid,andcompleteprotocols.
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
