SunScript™ReverseTranscriptaseRNaseH-isaengineeredreversetranscriptasebasedonthewellcharacterizedHIV-1RTwithhighThermostabilityandnoRnaseHactivity.IthasRNA-andDNA-dependentpolymeraseactivity.Thisenzymeisactiveupto85°C.TheeliminationofRNaseHactivityenablesthisenzymetoproducehighyieldsoffulllengthcDNAfromlongRNAmolecules,andthehighthermalstabilitypromotesreversetransriptionthroughcomplexsecondarystructures.

• Reversetransciptionattemperaturesashighas85°C
• HighsensitivityoverawideRNAofstartingRNAamounts
• ReversetranscriptionoflongmRNAs
• HighcDNAsynthesisrate

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Reversetransciptionattemperaturesashighas85°C

100ngoftotalRNAfromHeLacellswerereversetranscribedattheindicatedtemperaturesfor60minwithSunScriptReverseTranscriptasesandOligo(dT)18 primer,followedbyinactivationat95°Cfor10min.PresenceofGAPDHcDNAwasdetectedbyPCR(productlength305bp).

HighreactiontemperaturesareadvantageoustotranscribeRNAswithcomplicatedsecondarystructures.

 

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HighsensitivityoverawiderangeofRNAconcentrations

Sensitivitytest:RTreactionswereperformedat70°Cfor60min.Inactivationat95°Cfor10min.

Primerset:HumanGAPDH305bpset

1-1µgHumantotalRNAfromHeLacells
2-100ngHumantotalRNAfromHeLacells
3-10ngHumantotalRNAfromHeLacells
4-1ngHumantotalRNAfromHeLacells
5-100pgHumantotalRNAfromHeLacells

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TranscriptionoflongmRNAs

1-SunScript™RTRNaseH+replicate1
2-SunScript™RTRNaseH+replicate2
3-SunScript™RTRNaseH+replicate3
4-SunScript™RTRNaseH-replicate1
5-SunScript™RTRNaseH-replicate2
6-SunScript™RTRNaseH-replicate3

AssayforlongmRNAreversetranscription:3independentRTreactionswereperformedat65°Cfor60minusingOligo(dT)20 primers.Inactivationat95°Cfor10min.PCRPrimersets:mmNEB(>16kbfrom3’endofmRNA;mRNAtotallength22,5kb);mmDnah8(>8,5kbfrom3’endofmRNA;mRNAtotallength14,5kb)

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HighcDNAsynthesisrate

1µgoftotalRNAfromHeLacellswasreversetranscribedat65°CfortheindicatedtimeswithSunScript™ReverseTranscriptasesandOligo(dT)18primer,followedbyinactivationat95°C10min.

PCRPrimerset:hsHERC4033(>11kbfrom3’endofmRNA;mRNAtotallength15.3kb)

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ORDERINFORMATION

EachSunScript™RTRNaseH-contains:enzyme,5XReactionBufferandDTT.ThecompletemanualisavailableundertheManualtab.LucigenisanauthorizeddistributorofSygnisproductsintheUS.

SunScript™ReverseTranscriptaseRNaseH-isintendedformolecularBIOLOGyuseonlyandinvitrouseonly.Thisproductisnotintendedfordiagnosis,preventionortreatmentofadiseaseinhumanbeingsoranimals.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。