Lucigen/MMLV高效逆转录酶/RT80125K/25000单位

价格
¥2940.00
货号:RT80125K
浏览量:127
品牌:Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述

Optimizedreversetranscriptaseandbuffersystemfortheproductionoffull-lengthCDNA.

  • Synthesizefull-lengthcDNA(>15kb)Formerly from Epicentre
  • Amplifyfirst-strandcDNAfrompicogramamountsoftotalRNA
  • OptimizetheRTreactiontoyourspecificneedsbyusingthefirststrandcDNAsynthesisprimers,dNTPsandRNaseinhibitorofyourchoice(notincluded).

TheMMLVHPRTdemonstratessignificantlygreaterreversetranscriptaseactivitythanothercommerciallyavailableMMLVRTenzymes.Typically,just100unitsofMMLVHPRTarerequiredforfull-lengthcDNAsynthesiscomparedto200unitsofcompetitiveMMLVRTenzymes.MMLVHPRTincludesa10XReactionBuffer,optimizedforsynthesisoffull-lengthcDNAfromlongRNAtemplates,andDTT.Theenzyme,bufferandDTTarethesamecomponentsusedintheMMLVReverseTranscriptase1st-StrandcDNASynthesisKit.Byprovidingthesethreecomponentsindividually,youhavetheflexibilitytochoosedNTPs,RNaseInhibitors,etc.andoptimizetheRTreactionforyourspecificneeds.

Figure1.MMLVHPRTproducesfull-lengthcDNAfrommRNAlongerthan15kb. TotalRNAisolatedfromHeLacellswasreversetranscribedandthecDNAwasamplifiedbyPCR.Detectionofthe1.3-kbPCRampliconfromnearthe5´endofthemRNAdemonstratesfull-lengthreversetranscriptionofHERC1mRNA(A).AgarosegelanalysisofthePCRproductsshowsthe1.3-kbampliconfromthe5´endofthemRNA(B).LaneM,100bpDNAladder;lane1,no-RTcontrolreaction;lane2,PCRproductfromcDNAsynthesizedbyEpicentre"sMMLVHPRT.

TargetTranscript(Size)3´/5´Ratios
EpicentreMMLVHighPerformanceReverseTranscriptaseCompetitorI(RNaseH-MutantofMMLVRT)CompetitorP(MMLVRT)
ACTB(1,792b)0.91.71.2
GUSB(2,162b)1.06.12.5
TFRC(5,010b)5.512.111.3

Table1.3´/5´ratioanalysisofcDNAproducedbydifferentreversetranscriptaseenzymes. TotalcellularRNAfromHeLacellswasconvertedtocDNAusingthethreereversetranscriptaseenzymesindicatedinthetable.A3´/5´ratioequalto1.0meansthatequalamountsofPCRproductsareobtainedfromboththe3´and5´endofthecDNAandthereforeisagoodindicationthatthereversetranscriptasehasproducedafull-lengthcDNAcopyofthemRNA.


2A.

2B.

EpicentreMMLV1st-StrandcDNASynthesisKit

CompanyIRNaseH-minusMMLVRT

CompanyPMMLVRT

Figure2.cDNAproducedbyEpicentre"sMMLVHighPerformanceReverseTranscriptaseyieldsasignificantlyimproved3´/5´ratiothancompetitivereversetranscriptases. Theapproximately2160-baseHeLaβ-glucuronidasemRNA(GUSB)wasreversetranscribedintocDNAusingEpicentre"sMMLVHighPerfomanceReverseTranscriptaseandtwocompetitivereversetranscriptaseenzymes.PCRprimerpairstothe3´-endand5´-endofGUSBcDNAweresynthesizedandqPCR(SYBR® GreenIdyedetection)wasperformedusingeachprimerpairandtheGUSBcDNAsastemplates.The3´/5´ratiowascalculatedforeachasdescribedinthetext.(A).PCRampliconsfromthe3´endand5´endoftheGUSBcDNA.(B).qPCRquantificationgraphsfordetectingthe3´ampliconand5´ampliconofGUSBcDNAproducedbyEpicentre"sMMLVHighPerformanceReverseTranscriptaseKitandtwoothercommerciallyavailablereversetranscriptaseenzymes.

ORDERINFORMATION

MMLVHighPerformanceReverseTranscriptase,10XReactionBuffer,DTT. 美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。
  • 资质认证

    获得国家资质,权威认证!

  • 全国联保

    全国联保,官方无忧售后

  • 正规发票

    正规发票,放心购买

  • 签订合同

    签订合同,保障您的权益

/**/