- Lucigen特约代理_Lucigen
- Lucigen公司,LGC代理商,Lucigen经销商,LGC厂家直采,Lucigen官网...
- 请问大家中国北方工业公司（北京）笔试成绩出来 ...
- Lucigen 特约代理_文章
- epicentre中国代理,Lucigen中国代理,蓝景科信价格厂家:蓝景科信(...
- Frontiers | Limited Correlation of Shotgun Metagenomics...
- EconoTaq PLUS 2X Master Mix Lucigen
- 什么是lucigen产品 总代理中北林格为您略作讲解
- lucigen酶制剂产品分类及货号详情
- EconoTaq PLUS 2X Master Mix Lucigen
- 研究 STING 的 popular 工具
- 大肠杆菌电转化感受态细胞制备及转化方法 实验方法
Singlecellwholegenomeamplification(WGA)withlessbiasmakingthiskitideallysuitedfornextgenerationsequencingapplications.
- Highsensitivity:Typicalyieldisgreaterthan5µgwhenstartingwithasinglemammaliancell.
- Reducedamplificationbiasandnoprimerartefacts:Primer-freeamplificationmethodutilizesacombinationofTthPrimPolPrimase(tosynthesizeprimers)andPhi29DNApolymerase.
- Wellsuitedfornextgenerationsequencing:TestedinIlluminaandIonTorrentNGSworkflows.
- Easyandreliable
- InsensitivetoexternalDNAcontamination
- HowdoesTruePrime™Technologywork?
- Wholegenomeamplificationfrom5cells
- Absenceofprimerartefacts
- InsensitivitytoexternalDNAcontaminations
- Excellentgenomecoverage
HowdoesTruePrimetechnologywork?
Backtotop
Wholegenomeamplificationfrom5cells
Backtotop
Absenceofprimerartefacts
1pgofhumangenomicDNA(~1/6ofthecontentofonehuman/mammaliancell)hasbeenamplifiedusingeitherTruePrime™(TthPrimPol-basedMDA)orrandomprimedMDAreactions.Randomprimedreactionscontain20%ofsequencesthatcannotbemappedtoanyorganisminsequencedatabases.
Backtotop
InsensitivitytoexternalDNAcontaminations
Duetothehighsensitivityofsinglecellwholegenomeamplificationtechniquesthereisaconsiderabledangerofcontaminatingreactionswithnon-targetderivedDNA.Wethereforerecommendtotakeextremecarewhensettingupsinglecellamplificationreactionsaslaidoutinthehandbook.However,TruePrime™usershaveauniqueadvantagehere:TruePrime™doesnotacceptnon-denaturedDNAstrandsastemplateasreADIlyasrandomprimedMDAreactions.Therefore,contaminationscominginfromtheenvironmentetc.afterthedenaturationstepwillnothaveastronginfluenceonthecompositionofyourreactionoutput.WehaveshownthisbehaviourinanexperimentwherewesimulateanexternalDNAcontaminationbydeliberatelyaddinginyeastDNAtoadenaturedhumanDNAtemplate.YeastDNAisinathousand-foldexcessoverthetargetDNA(1pghumangenomicDNA):
WhereaswithTruePrime™thevastmajorityofthesequencesobtainedaretarget-derived,randomprimedMDAshowsamajorityofcontaminant-derivedsequences.
Backtotop
Excellentgenomecoverage
WehaveamplifiedandsequencedtheyeastgenomeusingTruePrime™andIlluminatechnology.Using2.5millionreadpairswecover99.4%ofthetotalyeastgenome.Shownisthecoveragewithnarrowwidthofthedistributioncurve.
Acoveragegraphofyeastchromosome7exemplifiesthemoreevencoverageobtainedbyTruePrime™MDA(middlebluelinerepresentsmeancoverage).
Backtotop
ORDERINFORMATION
EachTruePrime™SingleCellWGAKitversion2.0contains:BufferL1,DTT,BufferN,ReactionBuffer,dNTPs,Water,Enzyme1,Enzyme2,Control1,andControl2.ThecompletemanualisavailableundertheManualtab.LucigenisanauthorizeddistributorofSygnisproductsintheUS.TruePrime™SingleCellWGAversion2.0kitisintendedformolecularBIOLOGyuseonlyandinvitrouseonly.Thisproductisnotintendedfordiagnosis,preventionortreatmentofadiseaseinhumanbeingsoranimals. 美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
