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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/Ready Lyse™ 溶菌酶溶液/R1810M/10000000 U

Lucigen/Ready Lyse™ 溶菌酶溶液/R1810M/10000000 U

价格
￥9020.00
货号：R1810M
浏览量：127
品牌：Lucigen
服务
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商品描述

LyseGrampositiveorGramnegativebacteriaforproteinornucleicacidpurificationsfrombacteria

  • Epicentre ProductHighlyActive:Thisenzymeis200Xmoreactivethaneggwhitelysozyme,thusimprovingbacteriallysisandusinglessenzyme
  • Easy:Nosampleagitationorheatgenerationnecessaryduringlysiswhichhelpspreserveproteinfunction
  • FlexIBLe:Usuagevolumesareeasilyadjustedbasedonthescaleoftheexperiment

Applications

  • LysisofGram-negativeorGram-positivebacteria(Table1)forproteinpurification(Fig.1).
  • Purificationofnucleicacidsfrombacteria.

Ready-Lyse™LysozymeSolutionisarecombinantlysozymepreparation,derivedfromanonmammalian,nonaviansource,thatisusefulforthelysisofGram-negative(suchasE.coli)andGram-positive(suchasBacillussp.)bacteria.ThespecificactivityofReady-LyseLysozymeis200-foldhigherthanthespecificactivityofegg-whitelysozymeand,therefore,lesslysozymeisneededinareaction.Also,unlikeegg-whitelysozyme,Ready-LyseLysozymeSolutionisstableat-20°C,eliminatingtheneedtoprepareafreshsolutionforeachuse.TheuseofReady-LyseLysozymeresultsinhigheryieldsofproteinthancanbeobtainedwithstandardeggwhitelysozyme.

UnitDefinition:OneunitofReady-LyseLysozymeproducesadecreaseinA350of0.001perminuteat23°Cwitha0.5mg/mlsUSPensionoflyophilizedE.coliK802cellsin50mMTris-HCl(pH7.5).

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),0.1MNaCl,0.1mMEDTA,1mMDTT,and0.1%Triton®X-100.

Gram-NegativeGram-Positive

Escherichiacoli
Salmonellatyphimurium
Actinobacilluspleuropneumoniae
Rhodobactersphaeroides
Shewanellaputrefaciens
Flavobacteriaodoratum

Oerskoviaxanthinolytica
Bacillussubtilis

Table1.BacterialysedwithReady-Lyse™LysozymeSolution.

 

Ready-LyseFigure1.LysiswithReady-Lyse™Lysozymeincreasesyieldsofnucleicacids.500µgpermlofpHC79cosmidDNAwasincubatedfor15minutesat22°CinTEbuffer(25mMTris(pH8.0),10mMEDTA)containingeither5µg(30KU)permlofReady-LyseLysozymeor500µg(25KU)permlofeggwhitelysozyme.Thesolutionswerecentrifugedfor10minutesandthepelletswereresuspendedinTEbuffercontaining0.1%SDS.DNAinsupernatantsandpelletswasseparatedbyelectrophoresisina0.8%agarosegel.Approximately50%oftheDNAwaslostduetoprecipitationbyeggwhitelysozyme(EW),whileReady-LyseLysozyme(RL)causedminimalprecipitationlossesofDNAcomparedtocontrol(C)sampleswithoutlysozyme.Figure2.UseofReady-Lyse™LysozymeSolutiontorecoverrecombinantproteins.OnemlofinducedcellsfromarecombinantE.coliclonewaspelletedbymicrocentrifugationbeforeinductionandat1and3hoursafterinduction.Eachsamplewasresuspendedin50µlofcoldTEBGbuffer.OneµlofReady-LyseSolutionwasaddedtoeachsuspensionandthecellswereincubatedatroomtemperaturefor30minutes.Thecelldebriswaspelletedand10µlofthesupernatantwererunonanSDS-PAGEgel.Lane1,molecularweightMarkers;Lanes2-4,timepointsofinduction.Theinducedproteinisdesignatedbyanarrow.
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。