快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit/MBCL90310/10 Reactions

Lucigen/MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit/MBCL90310/10 Reactions

价格
￥4520.00
货号：MBCL90310
浏览量：127
品牌：Lucigen
服务
全国联保
正品保证
正规发票
签订合同
商品描述

ProduceamplifiedamountsofCDNAfromprecious,limitingcellsampleswithoutintroducingbias.

  • PerformRNAamplificationdirectlyfromculturedcelllysatesfollowedbycDNAsynthesisFormerly from Epicentre
  • Amplifywiththishigh-fidelity,linearRNAamplificationprocessthatpreservestherelativetranscriptabundanceofthesample.
  • Getmoredataoutofprecioussamples–uselessRNAformoreRT-qPCRreactions
  • ReADIlyandreproducIBLydetectevenlow-abundancetranscriptsfromaslittleasonecell.
  • NoneedtopurifyRNA:PerformRNAamplificationandcDNAsynthesisreactionsdirectlyfromcelllysates.
  • CollectsampleslessoftenandarchivecDNAforfutureuse,savingtimeandeffort.

Applications

  • AmplificationofmRNAdirectlyfromacelllysate,withouttheneedforpurifyingRNA,andconversionoftheamplifiedRNAtocDNA.
  • SignificantlyincreasingthenumberandsensitivityofqRT-PCRsfromverysmallnumbersofcells.
  • GenerationoflargeamountsofcDNAfromthemRNAofverysmallsamplesforarchivalpurposes.

TheMessageBOOSTER™cDNASynthesisfromCellLysatesKitenablestheusertoperformsensitiveqRT-PCRstudiesfromverysmallpopulationsofcells,evenfromaslittleasonecell.

ThekitamplifiesthecellularmRNAdirectlyfromacelllysate,withouttheneedtopurifytotalRNA,andthenconvertstheamplifiedRNAtocDNAthatisreadyforqPCR(Fig.1).AmplifiedcDNAisefficientlyproducedfromthelysatesof1to500cells.

Figure1(clicktoenlarge).AnoverviewoftheprocedurefortheMessageBOOSTER™cDNASynthesisfromCellLysatesKit.Akitreactionamplifiesthepoly(A)RNA(mRNA)directlyfromacrudecelllysatewithouttheneedforRNApurification.TheamplifiedRNAisthenreverse-transcribedtocDNAthatcanbedilutedupto1,000-foldforqPCR.
Figure2.AMessageBOOSTER™KitreactionproducessufficientcDNAfromasingle-celllysateforthousandsofsensitiveqPCRs.qPCRwasperformedusingundiluted(red),1:10diluted(green),1:100diluted(blue),and1:1,000diluted(purple)cDNAfromalysateofasingleNRKcell.Thelow-abundancePBGDtranscriptwasreadilydetected.

ORDERINFORMATION

Contents:QuickExtract™RNAExtractionSolution,MessageBOOSTER™T7-Oligo(dT)Primer,MessageBOOSTER™ReverseTranscriptionPreMix,RNaseInhibitor,MMLVReverseTranscriptase,MessageBOOSTER™DNAPolymerasePreMix,MessageBOOSTER™DNAPolymerase,MessageBOOSTER™cDNAFinishingSolution,MessageBOOSTER™InVitroTranscriptionPreMix,MessageBOOSTER™T7RNAPolymerase,MessageBOOSTER™T7TranscriptionBuffer,RNase-FreeDNaseI,RandomPrimers,RNaseH,ForwardControlPCRPrimer,ReverseControlPCRPrimer,100mMDTT,RNase-FreeWater,Poly(I). 美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。