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蚂蚁淘在线 / 品牌中心 / Lucigen / 卢西根/马斯特纯™ 酵母DNA纯化试剂盒/MPY80200/200纯化

卢西根/马斯特纯™ 酵母DNA纯化试剂盒/MPY80200/200纯化

价格
￥7420.00
货号：MPY80200
浏览量：127
品牌：Lucigen
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商品描述

ObtainhighyieldsofqualityDNAfromyeastwiththiseasytousekit

  • Epicentre ProductFast:ObtainpurifiedDNAinlessthan40minutes
  • FlexIBLe:PurifyDNAfromawidevarietyofyeastspecies,includingCandida,Saccharomyces,Pichia,andSchizosaccharomyces,andfilamentousfungisuchasAspergillus2andPenicillium
  • HighQualityDNA:PurifyhighmolecularweightgenomicDNAthatisreadytouseinmanymolecularBIOLOGyapplications,includingPCRamplification,restrictionendonucleasedigestion,Southernblotting,genomiclibrarypreparationandfungalidentificationandtyping
  • HighYields:PurifymoreyeastgenomicDNAthanothercommerciallyavailablekits
  • Simple:Noenzymaticlysis,beadbeating,columns,phenolorotherorganicsolventsareused

TheMasterPure™YeastDNAPurificationKitenablesefficient,high-yieldpurificationofhigh-molecular-weightDNAfromyeastandotherfungi.Theprotocolinvolvesnonenzymaticcelllysisat65°C,followedbyremovalofproteinbyprecipitation,andnucleicacidprecipitationandresUSPension.Nolyticase,proteolyticenzymes,orbead-beatingareusedintheprocedure.YeastgenomicDNAyieldsusingtheMasterPureKitaremuchhigherthanyieldsobtainedwithothercommerciallyavailablekits(Fig.1).1Theprotocolcanbeeasilyadjustedforlargerorsmallersamples,includingsingleyeastcolonies.1Therecoverednucleicacidcanbeuseddirectlyinmostapplications,includingPCRamplification.

References

  1. Hoffman,L.andMoan,E.(1998)EpicentreForum5(4),1.
  2. Jin,J.,Lee,Y.-K.andWickes,B.L.(2004)J.ClinMicrobiol.42,4293.

Figure1.ComparativeyieldsofDNAfromtheMasterPure™YeastDNAPurificationKitandcompetitorkits.DNAwasquantitatedspecificallywithHoechstfluorescentdye33258,whichgivesminimalfluorescencewithRNA.ThedatarepresenttheaverageDNAyieldsdeterminedbyfluorometryfromtwoexperimentswithS.cerevisiaeandC.albicans.TheMasterPureKitproducedupto17timesmoreDNAfromC.albicansand12timesmoreDNAfromS.cerevisiaethanotherkits.
Figure2.SizedistributionofDNAproducedfromtheMasterPure™Kit.Foreachkitindicated,a500-ngaliquotofpurifiedyeastDNAwasanalyzedbypulse-fieldgelelectrophoresisona1%agarosegel.Thegelwasstainedwithethidiumbromide.ThemajorityofDNAisolatedwiththeMasterPureKitwasestimatedtobeinthe40-to50-kbsizerange,whiledegradationtosmallerfragmentswasobservedwithotherkits.LaneM,lamBDaDNAladder;Lane4,T7DNA(40kb).

ORDERINFORMATION

Contents:YeastCellLysisSolution,MPCProteinPrecipitationReagent,TEBuffer,RNaseA. 美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。