卢西根/马斯特纯™ 完整的DNA和RNA纯化试剂盒/MC89010/10 DNA或5 RNA纯化*

价格
¥1520.00
货号:MC89010
浏览量:127
品牌:Lucigen
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商品描述

Quicklypurifyhighyieldsofhigh-molecular-weightgenomicDNA,totalcellularRNAorTotalNucleicAcid(TNA)withonekit

  • Epicentre ProductFast:PurifyTotalNucleicAcid(TNA),DNAorRNAin30–60minutes
  • Safe:Doesnotusehazardousphenol,chloroformorguanidine
  • HighPurity:A260/A280ratiosconsistentlybetween1.8and2.0
  • HighYields:Improvesyieldsbyavoidingtheuseofcolumnswhichoftenreducenucleicacidyields
  • Versatile:PurifyTNA,genomicDNA,totalRNA,FFPERNA,orbothgenomicDNAandtotalRNAfromasample
  • TotalRNARecovery:Purifybothlargeandsmall(e.g.,miRNA)RNAforRNA-SeqorqRT-PCR
  • Proven:HundredsofcitationsforpurificationofDNAandRNAfromdozensofsampletypesforuseinmanyapplications

TheMasterPure™CompleteDNAandRNAPurificationKitenablesrapid,highyieldpurificationofhigh-molecular-weightgenomicDNA,totalcellularRNAandTotalNucleicAcid(TNA)frommanydifferentsampletypes.

Thekitprocedureusesasafe,gentle,andscalablesalt-precipitationprotocol(Figure1)thateliminatestheneedforhazardouschemicalsandyield-reducingcolumns.Purificationreactionscanbescaleduporscaleddowndependingontheamountofnucleicacidneededandthesizeofthesample.MasterPureKitsforNucleicAcidpurificationsGram-positivebacteria,yeastDNAandbloodsamplesarealsoavailable.

Applications

PurificationofgenomicDNAortotalRNAformanyapplications,including:

  • Librarypreparationfornextgenerationsequencing(NGS)ofgenomicDNAandRNA
  • DNAmethylationstudiesusingIllumina®Infinium®HumanMethylationBeadChips
  • GenomicDNAandCDNAcloning
  • qPCRandqRT-PCR
  • Micrarrayanalyses(CGH,geneexpressionprofiling,etc)

Figure1.OverviewoftheMasterPure™CompleteKitprotocol.


Figure2.ComparisonofPCRsensitivityusingtemplatesobtainedusingtheMasterPure™CompleteDNAandRNAPurificationKitversusaspin-columnkit.PCRamplificationwasperformedafterextractionfromserialdilutionsofE.colicellsrangingfrom2×107(lanes1)to200(lanes6).LaneM,DNAMarker;lane7,negativecontrol.


Figure3.DNA,RNA,andtotalnucleicacid(TNA)purifiedfromdiversecellsourcesusingtheMasterPure™CompleteKit.M=kbladder.

美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。