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蚂蚁淘在线 / 品牌中心 / Lucigen / Lucigen/Fast Link&trade；DNA连接试剂盒/LK6201H/100连接

Lucigen/Fast Link&trade；DNA连接试剂盒/LK6201H/100连接

价格
￥5000.00
货号：LK6201H
浏览量：127
品牌：Lucigen
服务
全国联保
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商品描述
  • ClonewithspeedandefficiencyFormerly from Epicentre
  • TransformintoE.coliwithoutclean-upordesalting

Applications

  • Blunt-endandTAcloningofPCRproducts.
  • Ligationofnext-gensequencingadaptorstoblunt-endedDNA.
  • GenomicDNAcloningandsubcloning.
  • BAC/fosmidlibraryconstruction.
  • CDNAcloning.
  • Linkerligation.
TheFast-Link™DNALigationKitusesahigh-qualityligase(Fast-LinkT4DNALigase),thatwasclonedatEpicentreandformulatedtoprovideextremelyrapid,high-efficiencyDNAligation.Cohesive-endligationscanbeperformedin5minutesatroomtemperature.Incontrasttootherligases,itisnotnecessarytodesaltFast-Linkligationreactionspriortotransformationofelectrocompetentorchemicallycompetentcells.TheFast-LinkKitcanbeusedforroutineandhigh-throughputDNAcloning.
TypeofEndLigationTime%WhiteColoniesRecombinantsperµgDNA
Overhang5min.932.0x106
Blunt15min.714.4x105
PCRproductwith3´-Aoverhang1hr.681.2x104

Table1.Fast-Link™representativeresults.

Benefits

  • Cohesive-endligationsin5minutesatroomtemperature(Fig.1).
  • Blunt-endligationsin15minutesatroomtemperature(Fig.2).
  • LigationofPCRproductwithA-overhangsin1hourat16°C.
  • Highligationefficiency(Table1).
  • Desaltingofligationproductsisnotneededpriortotransformationofelectrocompetentorchemicallycompetentcells.
  • BACligationwithinserts>100kbcanbeperformedin4hours,insteadofovernightreactionsaswithotherligases.
Figure1.TimecourseforcohesiveendligationusingtheFast-Link™Kit.LamBDaHindIIIMarkerswereligatedusing2unitsofFast-LinkDNALigase(Lanes2-6).LaneM,Kilobaseladder;lane1,noenzyme.Figure2.Timecourseforblunt-endligationusingtheFast-Link™Kit.pUC19DNAdigestedwithPvuIIwasligatedusing2unitsofFast-LinkDNALigase(lanes1-6).LanesM,Kilobaseladder.

ORDERINFORMATION

Contents:Fast-Link™DNALigase,Fast-Link™10XLigationBuffer,10mMATP. 美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。