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蚂蚁淘在线 / 品牌中心 / Lucigen / 卢西根/E。cloni 10G ELITE电子电池>2×10^10 cfu/µg/60052-2/24 rxns（DUOs）

卢西根/E。cloni 10G ELITE电子电池>2×10^10 cfu/µg/60052-2/24 rxns（DUOs）

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￥5580.00
货号：60052-2
浏览量：127
品牌：Lucigen
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商品描述
  • Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.)
  • Optimizedgeneticsforhighyields: phageT1resistant,endonucleaseandrecombinationminus,blue/whitescreening-capable.
  • Availableinarangeofhightransformationefficiencies(5×109to4×1010cfu/µg).
  • Convenientpackagingoptions.

Lookingforchemicallycompetentversion?

Free Sample AvailableFreeSampleAvailable

 

Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™,DH10B™,JM109,TOP10,etc.anddirectlyreplacethemincloningprotocols.However,E.clonielectrocompetentcellsincorporateauniquemanufacturingtechnologythatincreasestransformationefficiency,recombinantyieldsandreliABIlity(Figures1and2),whiledecreasingcosts. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.

Choiceofstrain:

  • E.cloni10GCompetentCells.Libraryconstruction,cloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.
  • E.cloni10GF′CompetentCells.ContaintheF'plasmidforinfectionwithM13toproducessDNA.

Choiceofefficiency:

  • E.cloni10GSUPREMEElectrocompetentCells>4×1010cfu/µgpUCDNA
    SUPREMECellshavethehighesttransformationefficiencyavailablefromanysupplier.ChooseSUPREMECellsforthemostdemandingcloningsituations,suchasconstructionoflarge,highcomplexitylibrariesorcloningdifficulttargets,whichrequirethegreatestnumberoftransformantspossIBLe.
  • E.cloni10G&10GF´ELITEElectrocompetentCells>2×1010cfu/µgpUCDNA
    ELITECellshavetwicethetransformationefficiencycomparedto“ultrahighefficiency”cellsfromothersuppliers.ELITECellsprovidelargenumbersoftransformantsfromhard-to-clonefragmentsorlimitedDNAatalowerprice.
  • E.cloni10GCLASSICElectrocompetentCells>5×109cfu/µgpUCDNA
    CLASSICCellsarehighefficiencycellswithasubstantiallylowercostperreaction.Thesecellsarethemosteconomicalchoiceforstandardcloningandlibraryconstruction.10GCLASSICCellsareavailableinlargerpackagesizesforconvenientuseinhighervolumecloningapplications.

Custom CellsCustomCompetentCells

BulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.

Plasmidcloning.E.cloni10GSUPREMECellsproviderecombinantyieldshigherthanthehighestefficiencycellsofferedbyaleADIngsupplier(Figures1and2)

Recombinant Yields

 

Transformation Efficiency

Figure1.E.cloni10GSUPREMEElectrocompetentCellsconsistentlyoutperformed“ultrahighefficiency”cellsfromaleadingsupplier.Bothstrainsweretransformedwith10pgofpUC19(n=16).

 

Figure2.TransformationefficiencycomparisonofE.cloni10GSUPREMECells(Lucigen)andMegaDH10Bcells(Invitrogen).Competentcellsweretransformedaccordingtothemanufacturer’sinstructionswiththesamepUC19DNAcontrol(providedwiththeInvitrogencells)

Table1.E.cloniCompetentCellsforCloningandLibraryConstruction


E.cloniCellLines

TransformationEfficiency
(cfu/µgpUCDNA)

CloningMethylatedDNA

BAC,CosmidCloning

Blue/WhiteScreening

10GSUPREMEElectrocompetent

>4×1010

YES

YES

YES
withoutIPTGinduction

10GELITEElectrocompetent

>2×1010

YES

NO

YES
withoutIPTGinduction

10GCLASSICElectrocompetent

>5×109

YES

NO

YES
withoutIPTGinduction

10GF´ELITEElectrocompetent

>2×1010

YES

NO

YES
IPTGinductionrequired

Efficiencies

  • E.cloni 10GSUPREMEElectrocompetentCells > 4×1010 cfu/µgpUCDNA
  • E.cloni 10G&10GF´ELITEElectrocompetentCells > 2 × 1010 cfu/µgpUCDNA
  • E.cloni 10GCLASSICElectrocompetentCells > 5 × 109 cfu/µgpUCDNA 

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube),DUOs(2transformationspertube),orSixPacks(6transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。