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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
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https://www.ebiomall.com
商品描述
Description:RNase7,Human,ELISAkit
Antimicrobialproteins(AMP)areafundamentalelementoftheprimaryresponseagainstpathogens.AMPsaresmallendogenouscationicmoleculesexpressedbyphagocyticandepithelialcells.TheantimicrobialactivityofAMPsisdirectedtowardsabroadspectrumofpathogens,likeGram-positive&negativebacteria,viruses,yeastandfungi.AMPsaidininnateandadaptiveimmunityviadirectinactivationandbyimmunomodulatoryactivitylikeleukocytemigration.RNase7isanproteinsecretedbyavarietyofepithelialtissuesandisamemberoftheRNaseAsuperfamily.Thisfamilysharessequenceandstructuralsimilaritiessuchasconservedcysteineresiduesaswellasconservedhistidinesandalysineintheactivecentercatalysingtheribonucleaseactivity.RNase7isa14.5KDaproteinwithdistinctribonucleaseactivity.However,theantimicrobialactivityisindependentformtheribonucleaseactivitywhichmightbeassociatedwithantiviralactivity.Onapermolarbasis,RNase7isoneofthemostpotentAMPs.RNase7contributestothesterilityinseveralsystems.Itisdescribedtobeimportantinsterilityofthekidneyandurinarytractaswellasitscontributiontotheskinbarrierprotection.Intheurinarysystemitisconstitutivelyexpressedbytheintercalatedcellsintherenalcollectingtubulesandispresentintheurineatsuchlevelstokillbacteriaatbaseline.Intheskinitcontributestocontrolthegrowthofmicroorganismsontheskinsurface,andtheexpressionlevelscanbefurtherinducedundercontrolofproinflammatorycytokines.ThebactericidalactivityofRNase7hasbeenassociatedwithitsABIlitytobindandpermeatethebacterialcellmembrane.Thisrequiresclusteringoflysineresidues.RNase7isabletobindLPSandpeptidoglycans.Inocularsurface,signaltransductionassociatedwithRNase7expressionismediatedviaMAPKsbutnotNF-κBsignallingpathways.IL1βleadstoanincreasedexpressionofRNase7.
Specifications:
| Catalognumber | HK371-01 |
|---|---|
| Producttype | Assays |
| Quantity | 1x96det. |
| Standardrange | 78.1to5000pg/ml |
| Detectionlevel | 78.1pg/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Alias | RNase7,Ribonuclease7,Skin-derivedantimicrobialprotein2 |
| Application | ThehumanRNase7ELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanRNase7inserum,plasma,bronchoalveolarlavagefluid,urineandcellculturesupernatantsamples. |
| Disease | Infectiousdiseases,Nephrology |
| Principle | ThehumanRNase7ELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatofaplatewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.SamplesandstandardsareincubatedinmicrotiterwellscoatedwithantibodiesrecognizinghumanRNase7.BiotinylatedtracerantibodywillbindtothecapturedhumanRNase7.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanRNase7standards(log).ThehumanRNase7concentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| References | 1.KötenB,SimanskiM,GläserR,PodschunR,SchröderJ-M,etal.(2009)RNase7ContributestotheCutaneousDefenseagainstEnterococcusfaecium.PLoSONE4(7):e6424 2.SchwadererA,WangH,BartzJ,KlineJ,EichlerT,DeSouzaK,Sims-LucasS,BakerP,D(2013)Ribonuclease7,anantimicrobialpeptideup-regulatedduringinfection,contributestomicrobialdefenseofthehumanurinarytract.KidneyInt.83(4):615–625.3.BecknellB,EichlerT,BeceiroS,LiB,EasterlingR,CarpenterA,JamesC,McHughK,HainsD,Partida-SanchezS,SpencerJ.(2014)Ribonucleases6and7haveantimicrobialfunctioninthehumanandmurineurinarytract.KidneyInt.2014Jul30 |
