Description:

Description:UEV1A(ubiquitin-conjugatingenzymeE2variant1),alsoknownasUBE2V1orCROC-1(GenBankAccessionNo.NM_021988),a.a.2-170(end),withN-terminalFLAG-tag,MW=20kDa,expressedinE.coli.ActivityAssay:50?lreactionmix(4mMATP,5mMMgCl2,1mMDTT,UBE1(0.5?g),UEV1AorotherE2,Biotin-Ubiquitin(0.5?g)andSmurf2(2?g)in50mMTris,pH7.4)for30minat37°C.AddtoStreptavidin-coatedplatesfor30minat37°C.Incubatewithanti-Smurf2,thenHRP-labeledsecondaryantibody,andchemiluminescentdetection.Stability:>6monthsat-80°C.References:1.ThomsonTM,etal.(1998).FEBSLett.423(1):49-52.2.HauDD,etal.(2006).Biochemistry45(32):9866-77.3.MaL,etal.(1998).Oncogene17(10):1321-6.

Host:E.coli

Tag:N-terminalFLAG-tag

GINum:NM_021988

MW:20kDa

Format:aqueousbuffersolution

Formula:40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80?g/mlFLAGpeptide

ScientificCategory:UbiquitinConjugatingEnzyme(E2)

Species:human

AdditionalInformation:

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Name	UEV1A(UBE2V1)ActiveHumanRecombinantProtein
RelatedProductNames	UEV1A,UBE2V1,CROC-1,ubiquitin-conjugatingenzymeE2variant1
MolecularWeight	20kDa
Purity	40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80?g/mlFLAGpeptide
Source	E.coli
GINumber	NM_021988
Format	AqueousBufferSolution
Reactivity	Human
Storage	-80°C
IntendedUse	ResearchUseOnly