Description The Nano-CaptureLigand mouse IgG1 captures and immobilizes mouse IgG1 antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG1 subclass with high affinity and in a site-directed manner.

Specificity: Fc-fragment of mouse IgG1 antibodies Does not bind to: Goat, guinea pig, rabbit, rat, sheep, human, and macaque serum and to mouse IgG2a, IgG2b, IgG2c, and IgG3 Nano-CaptureLigands specificity overview

Conjugates: Biotin

Applications Mouse IgG1 capture to biosensors used in BLI and SPR Mouse IgG1 immobilization for ELISA

Advanced immobilization of antibodies Specific, stable, and site-directed immobilization of non-biotinylated mouse IgG1 antibodies to streptavidin/avidin

ChromoTek Nano-Traps通常用于pulldown目的蛋白及其与细胞提取物、细胞器的相互作用物。这些重组结合蛋白适用于快速、可靠、高效的分析以及为下游应用制备一步纯化蛋白等。Nano-Traps采用的抗体是基于alpaca的单域抗体片段(VHHS);具有分子量小(15Kda)、极端稳定(70℃仍保持稳定)，高亲和力(高特异性性结合GFP蛋白)，无普通抗体轻链与重链污染等一系列优点等优点;GFP抗体可以特异性结合绿色荧光蛋白的衍生物，如eGFP, wtGFP, GFP S65T，TagGFP，不与RFP衍生蛋白结合。   1. 产品类型：   蛋白质/肽标签   GFP-Trap 绿色荧光蛋白偶联系列   RFP-Trap 红色荧光蛋白偶联系列   Myc-Trap Myc标签偶联系列   GST-Trap GST标签偶联系列   MBP-Trap - NEW MBP标签偶联系列-新   特异性蛋白   Dnmt1-Trap Dnmt1偶联系列   Mdm4/ HdmX-Trap Mdm4/ HdmX偶联系列   MK2-Trap MK2偶联系列   p53 N-term-Trap p53 N-term偶联系列   p53 C-term-Trap p53 C-term偶联系列   PARP1-Trap PARP1偶联系列   Binding controls Binding control agarose beads 琼脂糖珠   Binding control magnetic agarose beads 磁性琼脂糖珠   Binding control magnetic particles 磁粉   2. 产品应用   免疫沉淀/共沉淀   质谱分析   酶活力检测   ChIP/RIP分析   3. 产品优点   对下游凝胶和质谱分析无重轻链污染   亲和力吸附作用极高   孵育时间短   超过1000篇相关文献报道   满足抗体验证的最高要求：结构和功能特征