Description Analyze and manipulate the intramolecular interaction between the N- and C-terminal domains of Androgen Receptor (AR) with ChromoTek Fluorescent-Two Hybrid (F2H^®) AR. Wild-type (wt), as well as several mutant AR forms can be investigated.

Specificity AR is expressed as two separate domains Bait: AR ligand binding domain (AR-LBD) fused to GFP Prey: AR N-terminal domain (AR-NTD) fused to RFP Bait and prey interact.

Applications Detect early steps of Androgen Receptor activation Real-time agonists and antagonists screening Visualized in live mammalian cells

Available AR variants AR-LBD: wt, W741L, F876L, T877A, F876L-T877A

Click on the left picture for a live cell analysis example Live-cell F2H imaging of reversible interactions between AR-LBD-GFP (green) and AR-NTD-RFP (red) in F2H-BHK cells

ChromoTek Nano-Traps通常用于pulldown目的蛋白及其与细胞提取物、细胞器的相互作用物。这些重组结合蛋白适用于快速、可靠、高效的分析以及为下游应用制备一步纯化蛋白等。Nano-Traps采用的抗体是基于alpaca的单域抗体片段(VHHS);具有分子量小(15Kda)、极端稳定(70℃仍保持稳定)，高亲和力(高特异性性结合GFP蛋白)，无普通抗体轻链与重链污染等一系列优点等优点;GFP抗体可以特异性结合绿色荧光蛋白的衍生物，如eGFP, wtGFP, GFP S65T，TagGFP，不与RFP衍生蛋白结合。   1. 产品类型：   蛋白质/肽标签   GFP-Trap 绿色荧光蛋白偶联系列   RFP-Trap 红色荧光蛋白偶联系列   Myc-Trap Myc标签偶联系列   GST-Trap GST标签偶联系列   MBP-Trap - NEW MBP标签偶联系列-新   特异性蛋白   Dnmt1-Trap Dnmt1偶联系列   Mdm4/ HdmX-Trap Mdm4/ HdmX偶联系列   MK2-Trap MK2偶联系列   p53 N-term-Trap p53 N-term偶联系列   p53 C-term-Trap p53 C-term偶联系列   PARP1-Trap PARP1偶联系列   Binding controls Binding control agarose beads 琼脂糖珠   Binding control magnetic agarose beads 磁性琼脂糖珠   Binding control magnetic particles 磁粉   2. 产品应用   免疫沉淀/共沉淀   质谱分析   酶活力检测   ChIP/RIP分析   3. 产品优点   对下游凝胶和质谱分析无重轻链污染   亲和力吸附作用极高   孵育时间短   超过1000篇相关文献报道   满足抗体验证的最高要求：结构和功能特征