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Product Name	14-3-3 sigma, 1-248aa, Human
Background	14-3-3 sigma ,also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E.coli and purified by using conventional chromatography techniques.
Species	Human
Synonyms	stratifin, Epithelial cell marker protein 1, HME1, YWHAS, SFN, 14-3-3 sigma
Host	E.coli
AA Sequence	MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
Tag	His-tag
Molecular Weight	18 kDa (160aa), confirmed by MALDI-TOF.
Form	Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol
Concentration	1 mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Applications	SDS-PAGE
NCBI	NP_006133
Storage	Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use	For Research Use Only
References	Benzinger A., et al. (2005) Mol Cell Proteomics. 4(6): 785-95.Wilker E W., et al. (2005) J Biol Chem. 280:18891-18898.

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