Mouse IgG2a Negative ControlCatalogue number: 02A1

Product Type	Single-Color Negative Control
Units	100 µg
Host	Mouse
Application	Flow Cytometry

BackgroundNegative control for Immunofluorescence staining with Pure, FITC, Biotin and PEofmousemonoclonalantibodies.Assessmentofnon-specific binding of mouse monoclonal antibodies to human cell surface antigens.

Synonyms: IgG2aunconjugated

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsConsult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood.PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze.WHOLE BLOOD: Add10µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters.Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser.Peak fluorescence emission is at 660 nm.RPE-Cy-5 +: Excites at 488nm and emits at 670nm.Store protected from light.

Functional Analysis: Flow Cytometry Staining

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Room Temperature

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

ReferencesCy-5Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Protein Reference(s)

Database Name: UniProt

Accession number: P01863

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

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