Mouse anti Human Calpain I, II (small subunit)Catalogue number: X1012

Clone	28F3
Isotype	IgG1
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Species reactivity	BovineHuman
Application	ELISAEnzyme ImmunoassayImmunoprecipitationWestern Blotting

BackgroundThe calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrationsofCa ++. Inadditiontotheubiquitouslyexpressedm-and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Synonyms: Mouse anti calpain I, II small subunit, p30

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Product

Product Form: Unconjugated

Formulation: Provided as a concentration of 1 mg/ml in 0.02M sodiumphosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsApplication: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.

Functional Analysis: Western Blotting

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Antibodies are stable for one year from purchase if stored frozen

Shipping Conditions: Room Temperature

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-482. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012

Protein Reference(s)

Database Name: UniProt

Accession number: P17655

Species Accession: Human

Safety Datasheet(s) for this product:NM_GlycerolNM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf/wp-content/uploads/SDS/SDS Antibodies with glycerol Nordic-MUbio.pdf

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