Mouse anti Human Cadherin-6Catalogue number: X1725M

Clone	2B6
Isotype	IgG1
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Species reactivity	HumanRat
Application	ImmunocytochemistryImmunohistochemistryWestern Blotting

BackgroundCadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.

Synonyms: K-Cadherin

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with affinity purified extracellular domain of human cadherin-6 fusion protein and SP2/0 mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsAntibody can be used for Western blotting (1:25-1:250), immunocytochemistry and immunohistochemistry on frozen sections using a PBS buffer containing 0.1 mM CaCl2and 0.1 mM MgCl2.Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Shimazui, T., et al. 'Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma,' Clin Cancer Res 1998, 4, 2419-24.2. Shimazui, T., et al. 'Alterations in expression of cadherin-6 and Ecadherin during kidney development and in renal cell carcinoma,' Eur Urol 2000, 38, 331-8.

Protein Reference(s)

Database Name: SwissProt

Accession number: P55285

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

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