Mouse anti Human Actin (N-Terminal)Catalogue number: X2672M

Clone	BA3R
Isotype	IgG2b
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Species reactivity	ChickenHumanMouseRabbitRat
Application	Dot blotELISAWestern Blotting

BackgroundActins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH,-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Source

Immunogen: -Actin N-terminal peptide-KLH conjugate

Product

Formulation: 10mM PBS, pH 7.2, with 0.05% Sodium Azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsWB (with ECL): 1:1000-10,000 dilutions (incubate for 1 hour at room temperature)IS: 1:500-2000 dilutionOptimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Recognizes native and denatured forms of β-Actin (~42kDa)

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1.Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L., Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.Nucleic Acids Res. 12:1687-1696(1984)2.Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T.,Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985)3.Procaccio V., Salazar G., Ono S., Styers M.L., Gearing M., Davila A., Jimenez R., Juncos J., Gutekunst C.-A., Meroni G., Fontanella B., Sontag E., Sontag J.-M., Faundez V., Wainer B.H. A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.Am. J. Hum. Genet. 78:947-960(2006) [PubMed: 16685646] [Abstract]

Protein Reference(s)

Database Name: SwissProt

Accession number: P60709

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

Nordic-MUbio开发和生产用于研发的高质量抗体试剂。我们所有的产品都是按照严格的ISO 9001质量准则生产的，我们的重点是开发针对细胞粘附，核蛋白和细胞骨架蛋白的抗体。Nordic-MUbio是开发用于斑马鱼研究抗体的领先抗体公司之一-我们目前已经验证了超过50种斑马鱼免疫荧光的抗体。在专有和合作开发计划中，Nordic-MUbio正在研究，测试和验证专利的抗体和抗原，用于肺癌和其他癌症领域的诊断应用。为了扩展其抗体和诊断应用产品的范围，Nordic-MUbio不断与不同的学术机构和研究小组保持密切联系，以选择其他机会：如果您对产品许可感兴趣，请发送电子邮件至info@nordicmubio.com。