Background:

Eachstainingperformedwithspecificmonoclonalantibodiesshouldbeparalleledbyastainingwithanappropriateistotypematchedcontrolantibody,inordertobeabletocontrolfornon-specificbinding.TheCOMBI-REAGENT-NegativeControlpermitstoestimatethedegreeofnon-specificbindingofisotypematchedimmunologbulinstoleukocytesviae.g.Fc-receptors.Itenablestheexperttosetflowcytometricparametersaccordingly.Resultsmustbeputwithinthecontextofotherdiagnostictestsaswellastheclinicalhistoryofthepatientbyacertifiedprofessionalbeforefinalinterpretation.Analysesperformedwiththisantibodyshouldbeparalleledbypositiveandnegativecontrols.Ifunexpectedresultsareobtained,whichcannotbeattributedtodifferencesinlaboratoryprocedures,pleasecontactus.

Product:

PBSpH7.2,1%BSA,0.05%NaN3

Applications:

DirectImmunofluorescence(StainingProcedure)Nordic-MubioFluorochromelabeledantibodiesaredesignedforusewitheitherwholebloodorisolatedmononuclearcell(MNC)preparations.Proposedstainingprocedureforwholebloodinshort:-Foreachsampleadd50µlofEDTAanti-coagulatedbloodtoa3-5mltube-Add20µloftheappropriateNordic-MUbiomonoclonalantibodyconjugate-Incubatethetubefor15minutesat4°Coratroomtemperatureinthedark-Add100µlNM-LYSE(Cat.No.GAS-003)toeachtubeandincubatefor10minutesatroomtemperature-Add3-4mlofdestilledwaterandvortex,incubatefor5-10minutesatroomtemperature-Centrifugetubefor5minutesat300g-AspiratesupernatantandresUSPendpelletin0.3mlofsheathfluid-Analyzeimmediatelyorstoresamplesat2-8°Cinthedarkandanalyzewithin24hoursFor“No-Wash”protocolpleaserefertowww.Nordicmubio.comProposedstainingprocedureforMNCinshort:-Carefullyadd20µlantibodyconjugateand50-100µlMNCtothebottomofatube-Vortexatlowspeedfor1-2seconds-Incubatefor15-30minutesat2-8°Coratroomtemperature-Centrifugetubesfor5minutesat300g-Removesupernatant,resuspendcellsin2-5mlofphosphatebufferedsaline(PBS)andcentrifugecellsagainfor5minutesat300g-Removesupernatantandresuspendcellsinsheathfluidforimmediateanalysisorresuspendcellsin0.5ml1%formaldehydeandstorethemat2-8°Cinthedark.Analyzefixedcellswithin24hours

Specificity:

Themonoclonalantibodieshavenoknownspecificityforhumanleukocytes.ItisrecommendedtousesimilaramountsoftestantibodyandisotypematchedCOMBI-REAGENTNegativeControlantibodyineachexperiment.

Storage:

Nordic-MUbiomonoclonalantibodyreagentscontainoptimalconcentrationsofaffinity-purifiedantibody.ForstABIlityreasonsthismonoclonalantibodysolutioncontainssodiumazide.Thesereagentsshouldbestoredat2-8°C(DONOTFREEZE!)andprotectedfromprolongedexposuretolight.Stabilityofthereagent:Pleaserefertotheexpirydateprintedontothevial.Theuseofthereagentaftertheexpirationdateisnotrecommended.

Caution:

Forprofessionalusersonly.Thisreagentcontainssodiumazide.Toavoidthedevelopmentofhazardousconditions,reagentscontainingazideshouldbedilutedinrunningwaterpriortobediscarded.SimilartotheworkwithotherBIOLOGicalproducts,properhandlingproceduresarerecommended. Nordic-MUbio开发和生产用于研发的高质量抗体试剂。我们所有的产品都是按照严格的ISO 9001质量准则生产的，我们的重点是开发针对细胞粘附，核蛋白和细胞骨架蛋白的抗体。Nordic-MUbio是开发用于斑马鱼研究抗体的领先抗体公司之一-我们目前已经验证了超过50种斑马鱼免疫荧光的抗体。在专有和合作开发计划中，Nordic-MUbio正在研究，测试和验证专利的抗体和抗原，用于肺癌和其他癌症领域的诊断应用。为了扩展其抗体和诊断应用产品的范围，Nordic-MUbio不断与不同的学术机构和研究小组保持密切联系，以选择其他机会：如果您对产品许可感兴趣，请发送电子邮件至info@nordicmubio.com。