Background:

Inimmunoelectrophoresisagainstpooledserumandenrichedserumproteinsfractionsprecipitationcanbeobservedofnotlessthan20individualproteinscomponents.InprecipitatingtechniquesasimmunoelectrophoresisandrADIalimmunodiffusion(Ouchterlony)toidentifytheserumproteinpattern,orthepresenceorabsenceofanindividualcomponent.Toevaluatethepurityofanisolatedserumproteinincludingimmunoglobulins.Sinceimmunoprecipitationdependsonacorrectantigen/antibodyconcentrationratio(zoneofequivalence)inthegelmedium,theproteinanalysisbyimmunoelectrophoresisofserumoranyotherBIOLOGicalfluidorproteinfractionshouldincludedifferentproportionsofthereactants.ItisnotpossIBLetoobtainanoptimalproteinpatterninasingleanalysis.Theelectroendosmosiseffectofdifferenttypesofagaronproteinswithadifferentnetchargecanbeusedtooptimizetheresolutionpowerofthetestsystem.AgarNordicNr.2containssufficientpositivelychargedionstooptimizetheresolutionoftheproteinsinthebeta-gammaregions,whilethealpharegionswillbecomemoredense.Highlypurifiedagar(AgarNordicnr.1)withlowelectroendosmosisfavourstheresolutionoftheproteinsinthealpharegions,whilethemajorcomponentsinthebeta-gammaregioncanstillbeidentified.

Source:

Pooledwholedogserumandpartlypurifiedserumfractions.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Delipidated,heatinactivated,lyophilized,stablewholeantiserum.Nopreservativeadded.TotalproteinandIgGconcentrationsintheantiserumarecomparabletothoseofpoolednormalgoatserum.Noforeignproteinsadded.Reconstitutethelyophilizedantiserumbyadding1mlsteriledistilledwater.

Applications:

Precipitationassays.Inimmunoelectrophoresisuse2μlserumorequivalentagainst120μlantiserum.Indoubleradialimmunodiffusion(Ouchterlony)usearosettearrangementwith10μlantiserumin3mmdiametercenterwelland2μlserumsamples(neatandseriallydilutedin2mmdiameterperipheralwells.Differentbleedingsoftheimmunizedanimalsarepooledtoobtainabroadspectrumbalancedagainstthevaryingconcentrationsoftheindividualserumproteincomponents.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoanimalproteinssincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.ThisantiSerumhasnotbeenadsorbedforsuchcross-reactivity.Consequentlyitisnotspecies-specific.

Specificity:

PrecipitatingpolyclonalGoatantiSerumtoDogSerumproteins

Storage:

Thelyophilizedantiserumisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Dilutionsmaybepreparedbyaddingphosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheantiserum.Dilutedantiserumshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic-MUbio开发和生产用于研发的高质量抗体试剂。我们所有的产品都是按照严格的ISO 9001质量准则生产的，我们的重点是开发针对细胞粘附，核蛋白和细胞骨架蛋白的抗体。Nordic-MUbio是开发用于斑马鱼研究抗体的领先抗体公司之一-我们目前已经验证了超过50种斑马鱼免疫荧光的抗体。在专有和合作开发计划中，Nordic-MUbio正在研究，测试和验证专利的抗体和抗原，用于肺癌和其他癌症领域的诊断应用。为了扩展其抗体和诊断应用产品的范围，Nordic-MUbio不断与不同的学术机构和研究小组保持密切联系，以选择其他机会：如果您对产品许可感兴趣，请发送电子邮件至info@nordicmubio.com。