Mouse anti Human CD103Catalogue number: 1031

Clone	AX.14
Isotype	IgG1
Product Type	Single-Color Reagent
Units	100 µg
Host	Mouse
Species reactivity	Human
Application	Flow Cytometry

BackgroundThe CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cellsand on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is theE integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely,peripheral blood lymphocytes.CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.

Synonyms: CD103

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsPBMC: Add 10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS andanalyze.WHOLE BLOOD:Add 10 µl of MAB/100 µl of whole blood.Mix gently and incubate for 15 minutes at room temperature (20° C).Lyse the whole blood. Wash once with PBS and analyze or fix with0.5% v/v of paraformaldehyde in PBS and analyze.See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN:(APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters.Laser excitation is at 633 nm with a Helium Neon(HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser.Peak fluorescence emission is at 660 nm.

Functional Analysis: Flow Cytometry Staining

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Room Temperature

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Cepek K., Wong D. Brenner M., and Springer T., Leukocyte Typing V val. G p 1666-67 (1995).

Protein Reference(s)

Database Name: UniProt

Accession number: P38570

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

Nordic-MUbio开发和生产用于研发的高质量抗体试剂。我们所有的产品都是按照严格的ISO 9001质量准则生产的，我们的重点是开发针对细胞粘附，核蛋白和细胞骨架蛋白的抗体。Nordic-MUbio是开发用于斑马鱼研究抗体的领先抗体公司之一-我们目前已经验证了超过50种斑马鱼免疫荧光的抗体。在专有和合作开发计划中，Nordic-MUbio正在研究，测试和验证专利的抗体和抗原，用于肺癌和其他癌症领域的诊断应用。为了扩展其抗体和诊断应用产品的范围，Nordic-MUbio不断与不同的学术机构和研究小组保持密切联系，以选择其他机会：如果您对产品许可感兴趣，请发送电子邮件至info@nordicmubio.com。