Background:

ThereactivityoftheantiserumisdirectedtotheFcsubunitoftheIgGmoleculewhichexpressesstrictisotypic(class)specificity.Itdoesnotreactwithanynon-Igproteinindogserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.InimmunocytochemicalandimmunohistochemicaluseforthedetectionofIgGatthecellularandsubcellularlevelbystainingofappropriatelytreatedcellandtissuesubstrates;todemonstratecirculatingIgGantibodiesinSEROdiagnosticmicroBIOLOGyandautoimmunediseases;toidentifyaspecificantigenusinganreferenceantibodyofgoatoriginknowntobeoftheIgGisotypeinthemiddlelayeroftheindirecttestprocedure;innon-isotopicassaymethodology(e.g.ELISA)tomeasureIgGindogserumorotherbodyfluids.Asasecondstepanavidinorstreptavidinconjugateoftheuser’schoicehastobeused.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

PurifiednormalIgGisolatedfrompooleddogserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Biotin-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

Specificity:

Biotin-conjugatedIgGfractionofpolyclonalGoatantiSerumtoDogIgG,Fcspecific

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic-MUbio开发和生产用于研发的高质量抗体试剂。我们所有的产品都是按照严格的ISO 9001质量准则生产的，我们的重点是开发针对细胞粘附，核蛋白和细胞骨架蛋白的抗体。Nordic-MUbio是开发用于斑马鱼研究抗体的领先抗体公司之一-我们目前已经验证了超过50种斑马鱼免疫荧光的抗体。在专有和合作开发计划中，Nordic-MUbio正在研究，测试和验证专利的抗体和抗原，用于肺癌和其他癌症领域的诊断应用。为了扩展其抗体和诊断应用产品的范围，Nordic-MUbio不断与不同的学术机构和研究小组保持密切联系，以选择其他机会：如果您对产品许可感兴趣，请发送电子邮件至info@nordicmubio.com。