14-100 kDa Protein Marker. Protein Marker is composed of seven prestained proteins ranging from 14 kDa to 100 kDa. The protein of 50 kDa band is covalently coupled to orange dye. The protein of 14 kDa is covalently coupled to yellow dye. The other five proteins are covalently coupled to blue dye. After SDS-PAGE and transferred to PVDF or NC membrane, clear color protein bands are gained and show the direction at the same time. Five blue bands, one orange band and one yellow band. MW range from 14 kDa to 100 kDa. Ready-to-use format, no heating and reductant are required.

Target	14-100 kDa Protein Marker
Storage	Store at -20°C. Stable for up to 2 years from date of receipt.
Molecular Weight	14-100 kDa
Concentration	Approximately 2 µg/5 µl for each band
Buffer	100 mM Tris-HCl (pH 6.8), 5 mM EDTA, 10 mM DTT, 10% glycerol, 1% SDS, 0.01% phenol red.
Directions for use	Mix well before use. Use 5 µl/well for small gels or 10 µl/well for larger gels. Suggested Electrophoresis Conditions:Carry out electrophoresis at 200 V for 50 minutes, then transfer to membrane at 200 mA for 3 hours.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 14-100 kDa Protein Marker RNA Interference Products RNA干扰（RNA interference, RNAi）是指在进化过程中高度保守的、由双链RNA（double-stranded RNA，dsRNA）诱发的、同源mRNA高效特异性降解的现象。基因沉默，主要有转录前水平的基因沉默(TGS)和转录后水平的基因沉默(PTGS)两类:TGS是指由于DNA修饰或染色体异染色质化等原因使基因不能正常转录;PTGS是启动了细胞质内靶mRNA序列特异性的降解机制。有时转基因会同时导致TGS和PTGS。 由于使用RNAi技术可以特异性剔除或关闭特定基因的表达，（长度超过三十的dsRNA会引起干扰素毒性）所以该技术已被广泛用于探索基因功能和传染性疾病及恶性肿瘤的治疗领域。