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商品描述
30-200 kDa Protein Marker. Protein Marker IV is composed of six unstained recombinant proteins 40 kDa, 60 kDa, 80 kDa, 100 kDa, 150 kDa, 200 kDa and one blue prestained recombinant protein 30 kDa. Prestained protein is obvious to monitor electrophoresis. 100 kDa band is a double intensity band for convenient identification of protein molecular weight. MW range from 30 kDa to 200 kDa. Ready-to-use format, no heating and reductant are required.
Research Articles on 30-200 kDa Protein Marker
RNA Interference Products
RNA干扰(RNA interference, RNAi)是指在进化过程中高度保守的、由双链RNA(double-stranded RNA,dsRNA)诱发的、同源mRNA高效特异性降解的现象。基因沉默,主要有转录前水平的基因沉默(TGS)和转录后水平的基因沉默(PTGS)两类:TGS是指由于DNA修饰或染色体异染色质化等原因使基因不能正常转录;PTGS是启动了细胞质内靶mRNA序列特异性的降解机制。有时转基因会同时导致TGS和PTGS。
由于使用RNAi技术可以特异性剔除或关闭特定基因的表达,(长度超过三十的dsRNA会引起干扰素毒性)所以该技术已被广泛用于探索基因功能和传染性疾病及恶性肿瘤的治疗领域。
| Target | 30-200 kDa Protein Marker |
| Storage | Store at -20°C. Stable for up to 2 years from date of receipt. |
| Buffer | 50 mM Tris-HCl, pH 6.8, 5 mM EDTA, 10 mM DTT, 10% glycerol, 1% SDS, 0.01% phenol red. |
| Concentration | 30 kDa band: 2 µg/5 µl100 kDa band: 1 µg/5 µlAll other bands: 0.5 µg/5 µl |
| Directions for use | Mix well before use. Use 5 µl/well for small gels or 10 µl/well for larger gels. Suggested Electrophoresis Conditions:Carry out electrophoresis at 200 V for 50 minutes. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |


