Annexin V can be used as one of the most sensitive indicators to detect the early-stage apoptosis. In normal live cells, phosphatidylserine (PS) is located only on the inner face of plasma membrane. However, in the early-stage apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, thus exposing PS to cell surface. Annexin V is a Ca 2+ -dependent phospholipid- binding protein that has a high affinity for PS. Therefore, Annexin V can identify early-stage apoptosis by binding to PS exposed on the outer leaflet. Annexin V can be labeled with green fluorophore (FITC), the labeled Annexin V-FITC is used as a probe to detect the occurrence of apoptosis by flow cytometry or by fluorescence microscopy. Propidium iodide (PI) is a nucleic acid binding dye, and it is impermeant to live cells with intact membrane and early apoptotic cells. However, PI can stain the nucleus through permeating to cell membrane of mid-stage or late-stage apoptotic cells and necrosis cells, which show red fluorescence under certain laser excitation.

Target	Annexin V-EGFP/PI Cell Apoptosis
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Research Articles on Annexin V-EGFP / PI Cell Apoptosis Detection Kit RNA Interference Products RNA干扰（RNA interference, RNAi）是指在进化过程中高度保守的、由双链RNA（double-stranded RNA，dsRNA）诱发的、同源mRNA高效特异性降解的现象。基因沉默，主要有转录前水平的基因沉默(TGS)和转录后水平的基因沉默(PTGS)两类:TGS是指由于DNA修饰或染色体异染色质化等原因使基因不能正常转录;PTGS是启动了细胞质内靶mRNA序列特异性的降解机制。有时转基因会同时导致TGS和PTGS。 由于使用RNAi技术可以特异性剔除或关闭特定基因的表达，（长度超过三十的dsRNA会引起干扰素毒性）所以该技术已被广泛用于探索基因功能和传染性疾病及恶性肿瘤的治疗领域。