\"如果最后证明文章的东西是虚构的,我表示非常遗憾,我参与到里面了,我不会逃避这个责任。”针对目前各方对于NgAgo技术的质疑,浙江大学医学院基础医学系研究员、韩春雨论文的共同专利申请人、论文的另一合作者沈啸回应,\"这次事件无论结果如何,是个标杆性事件,对各界都有很大的启迪。政府、媒体、科研单位以
重组DNA的分离、克隆与测序实验手册-6Electroporation ProtocolPreparation of Electro-competent Cells:1. Grow XL1-Blue cells on a tetracycline plate (20 ug tet/ml of LB agar)2. Inoculate 3
韩春雨论文共同作者沈啸首次公开回应\"如果最后证明文章的东西是虚构的,我表示非常遗憾,我参与到里面了,我不会逃避这个责任。”针对目前各方对于NgAgo技术的质疑,浙江大学医学院基础医学系研究员、韩春雨论文的共同专利申请人、论文的另一合作者沈啸回应,\"这次事件无论结果如何,是个标杆性事件,对各界都有很大的启迪。政府、媒体、科研单位以
RNA酶活性的控制为了获得高质量的真核细胞mRNA, 必须使用RNA酶的抑制剂或采用下述的破碎细胞和灭活RNA酶同步进行的方法,最大限度地降低细胞破碎过程中所释放的RNA酶的活性。同时,避免偶然引入实验室内其他潜在的痕量RNA酶也很重要。下面列举避免RNA酶法染问题的一些注意事项。大多数有经验的研究者并不拘泥于这些注
Organotypic raft culture of primary human keratinocytes (PHKs)Organotypic raft culture of primary human keratinocytes (PHKs)NotesUse 106keratinocytes per raft.Typical ProtocolTrypsinize the PHKs off the fla
Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast ...Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in ArabidopsisAs part of a project to analyze chloroplast functi
Bromodeoxyuridine ImmunohistochemistryIntroduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
Combined Flow Cytometric Measurement of Two Cell-Surface AntigensINTRODUCTIONFlow cytometry is frequently used to assess nucleic acid contentin individual cells. Based on DNA content alone, however, cells 
流式应用精选——微核检测(体内&体外)注:点击文章名称查看详情。文章名称样本仪器型号备注AssessmentoftheGenotoxicPotentialofAzidothymidineintheComet,Micro
Enzyme Kinetics assay of the WTTo assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media
组织学——组织制备·Histological techniques(William H. Heidcamp)Very detailed guide to histological technique
immunofluorescence of rabbit anti-murine VEGF by Peprotech实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.实验步骤The following protocol used normal muri
B 淋巴细胞膜表面免疫的检查实验概要本实验介绍了用直接免疫荧光法检查B 淋巴细胞膜表面免疫球蛋白(SmIg)的基本原理和操作步骤。实验原理SmIg 是B 细胞的抗原识别受体,也是B 细胞特异的表面标志,用直接免疫荧光法可检查出SmIg。用荧光素标记的抗Ig 抗体,在一定条件下与淋巴细胞混合,荧光素标记的
溶菌酶的制备、纯化和鉴定实验概要制备、纯化和鉴定溶菌酶实验原理溶菌酶,全称为1,4-β-N-溶菌酶,又称粘肽N-乙酰基胞壁酰水解酶,属于α-乳白蛋白家族。人们对溶菌酶的研究始于上世纪初,英国细菌学家Fleming发现人的唾液、眼泪中存在有溶解细菌细胞壁的酶,因其具有溶菌作用,故命名为溶菌酶;此后人们在微生物、植物、无脊椎动
Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana)Agrobacterium -mediated transformation of Nicotiana species, namely, Nicotiana tobaccum and Nicotiana benthamiana , using leaf disks as the target e
Agrobacterium growth and transformationGrowth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic
Blocking cDNA arrays printed on amine slidesDepartment of Crop SciencesCollege of Agricultural, Consumer, and Environmental SciencesUniversity of Illinois at Urbana-ChampaignPurpose:After
Optimized Method for the Preparation of Rodent Testicular Cells-1Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
TACI and BCMA stimulation of B cell immune responses.TACI and BCMA signal transduction pathway that enhances cell survival APRIL and BAFF (also called TALL-I and BLyS) are TNF family members that act as
Lightscanner 和Lightscanner32 非标记探针法基因分型Lightscanner 和Lightscanner32 非标记探针法基因分型载脂蛋白(ApoE)多重实验密码子112(T C)密码子158(C T)实验背景 载脂蛋白(ApoE)是一种糖蛋白,包括299个氨基酸,在身体调节胆固
Leaf GUS Assay实验概要a protocol forLeaf GUS AssayThis protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs
溶血空斑试验实验概要本实验介绍了溶血空斑试验即小室液相法(Test of Plague Forming Cell)的基本原理及操作步骤。实验原理溶血空斑试验,又称空斑形成细胞(Plague Forming Cell,PFC)试验,是一种体外检测抗体形成细胞的方法。将一定量洗涤过的绵羊红细
为何要关注抗体药物的糖基化修饰?在众多的蛋白质翻译后修饰中,糖基化修饰是最重要和最复杂的修饰之一,也是评价抗体的关键质量属性之一。单抗药物功能的实现与其糖基化修饰密切相关,糖基化修饰会影响蛋白的性能,如构象、稳定性、溶解度、药物代谢动力学、活性及免疫原性。本文中,笔者就糖基化及其对抗体药物的稳定性/半衰期、安全性及生物活性进
Fungal DNA IsolationFungal DNA IsolationSaghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) PNAS 81:8014-8018DNA successfully isolated from fungal species
使用MD SoftMax Pro 7软件进行平行线分析和相对活性..(一)使用MD SoftMax Pro 7软件进行平行线分析和相对活性评价介绍在生物实验中要经常要使用到的平行线分析(PLA)方法,当无法直接检测一个生物制品的活性而只能通过检测其产生的效果时,PLA方法通常会被用来进行效应曲线的比较来获得此制品的效果(Figure1)。如果对于两种物质其生物响应相似或者
典奥生物:专业代理,专业服务 2013年6月6日,实验室自动化与筛选协会2013亚洲会展在上海金茂君悦大酒店盛大开幕。本次年会围绕\"药物研发和实验室技术”这一主题展开,吸引了来自全球药物研发领域的相关科研人员参会。典奥生物作为生命科研领域仪器设备软硬件、耗材的专业服务的代理公司在本次展会上展出了Echo 500系列 Immunofluorescent Staining of Drosophila Larval Brain Tissue实验概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
Isolation of Genomic DNA from Tissue Using ChargeSwitch® Technology实验概要The ChargeSwitch® gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or
骨组织β-gal免疫染色实验技术方法ß-gal Staining:Reagents:0.1M Phosphate Buffer (pH 7.3):0.1M Sodium Phosphate monobasic115ml0.1M sodium phosphate dibasic385mlTotal Volume500 mlFix sol
Cr Release Cytotoxicity assayDescriptionCytotoxic activities of mNK cells are examined in 51Cr release assay from target cellsProcedureEffector cells (mNK cells) are seeded
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