ProductDescriptionSize:100μlAbType:pReactivity:AllImmunogen:NitratedKLHHost:rabbit

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ProductDescriptionTherabbit-derivedanti-3NTantibodyispurifiedbynitratedtyrosineagaroseaffinitychromatography.Itspecificallyrecognizesproteinswithtyrosinenitratedresiduesbutnotthetyrosinephosphorylatedornon-modifiedtyrosineresidues.BesidescommonapplicationsinWesternblottingandimmunofluorescence,theantibodyhasbeenwellutilizedforproteomicscreeningtotyrosinenitratedsubstratesfromprotease-digestedproteinsbothinprokaryoticandeukaryoticcells.Source/PurificationThisproductisproducedbyimmunizingrabbitswithnitratedKLH.TheantiserumwasimmunoaffinitypurifiedbyproteinAandfollowedwithnitrotyrosinecontainingpeptides.SpecificityAlltyrosinenitratedproteinsexpectedRecommendedApplicationsELISA,WB,IP*WB=Westernblotting; IP=ImmunopreciptationRecommendedantibodydilutionWB:1:1000ImportantForWesternblotting,incubatemembranewithdilutedantibodyin5%BSA,1xTBS,0.1%Tween-20at4℃withgentleshaking,overnight.Useatanassaydependentconcentration.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.Notyettestedinotherapplications.Dotblottinganalysis onindicatedamountofpeptidesusing3-nitrotyrosinerabbitpAb.Thelistofpeptidesisincludedinthetableontheright.Westernblotanalysis on30μgofextractsfromHeLaandMEFwholecelllysates,untreatedortreatedwithperoxynitrite(3mM,30mins)orpervanadate(3mM,30mins),usinganti-3-nitrotyrosineAntibody(FigureB)oranti-phospho-TyrosinerabbitpAb(PTM-702)(FigureC).ScientificDescriptionNitrotyrosineisaproductoftyrosinenitrationmediatedbyreactivenitrogenspeciessuchasperoxynitriteanionandnitrogendioxide.ItisverystableandsoissuitableforanalysisinBIOLOGicalsamplesuchasplasma,lungaspirants-BALF(Bronchoalveolarliningfluid)andurine.IncreasednitrotyrosinelevelshavebeenassociatedwithseveralphysiologicalandpathologicalconditionsandisconsideredaMarkerofNO-dependent,reactivenitrogenspecies-inducednitrativestress.ResearchshowsthatnitrotyrosinelevelscanbereducedbyN-acetylcysteinewhichisaprecursortoglutathione,oneofthebody’sprimaryendogenousantioxidants.Storage&StABIlityTheantibodyisdeliveredinlyophilizedpowder.Uponreceipt,pleasecentrifugetheantibodyat12,000xgfor20seconds,andthenreconstituteitwiththeantibodystabilizerprovided.ThestabilizercontainsPBS,50%glycerol,and0.01%sodiumazide.Pleasestoretheantibodyat-20°C.Avoidrepeatedfreeze/thawcycles.Antibodyisstablefor12monthsfromdateofreconstitution.Equilibratetheantibodytoroomtemperaturefor2minutesandmixbygentlypipettingbeforeuse.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals. ICL抗体抗ASFV抗体（小鼠-Clone＃10G5）-单克隆货号：MASF-5A-10G5 主持人：鼠标 反应性：ASFV-P72 目标：非洲猪瘟病毒1.0毫克$ 375.00抗牛肝珠蛋白抗体（山羊）-亲和纯化货号：GHPT-10A-Z 主持人：山羊 反应物种：牛，牛 目标：单珠蛋白0.1毫克$ 145.00抗牛肝珠蛋白抗体（小鼠-Clone＃1B5）-单克隆货号：MHPT-10ALY-1B5 主持人：鼠标 反应物种：牛，牛 目标：单珠蛋白1.0毫克$ 375.00抗牛IFN-γ抗体（小鼠-Clone＃1B11）-单克隆货号：MFNG-10ALY-1B11 主持人：鼠标 反应物种：牛，牛 目标：IFNγ1.0毫克$ 450.00抗牛IFN-γ抗体（小鼠-Clone＃2E6）-单克隆货号：MFNG-10ALY-2E6 主持人：鼠标 反应物种：牛，牛 目标：IFNγ1.0毫克$ 450.00抗牛IgG h + 1抗体（兔）-亲和纯化货号：RGHL-10A 主持人：兔子 反应物种：牛，牛 目标：IgG h + 11.0毫克$ 55.00抗牛SAA抗体（小鼠-Clone＃1F12）-单克隆目录编号：MSAA-10A-1F12 主持人：鼠标 反应性：牛 目标：血清淀粉样蛋白A1.0毫克$ 375.00抗牛SAA抗体（小鼠-Clone＃1G6）-单克隆目录编号：MSAA-10A-1G6 主持人：鼠标 反应性：牛 目标：血清淀粉样蛋白A1.0毫克$ 375.00抗牛SAA抗体（小鼠-Clone＃2D7）-单克隆目录编号：MSAA-10A-2D7 主持人：鼠标 反应性：牛 目标：血清淀粉样蛋白A1.0毫克$ 375.00抗牛全血清抗体（兔）-抗血清目录编号：RWS-10 主持人：兔子 反应物种：牛，牛 目标：全血清1.0毫升$ 35.00