FibroblastsCataloguenumber:X1004

ProductType	ControlLysate
Units	100µl
Application	WesternBlotWesternBlotting

BackgroundTotalcellIysatesareusefulasbothpositiveandnegativecontrolsinimmunoblotting.A431,ahumanepidermoidcarcinomacellline,andtheEGF-stimulatedA431Iysatesareusedasnegativeandpositivecontrols,respectively,whenstudyingthephosphorylationcascadeinitiatedbyligandbindingtoreceptortyrosinekinases.BothA431andnormalhumanfibroblastsarevaluablepositivecontrolsforantibodiestoproteinsexpressedinthesecells.Themousefibroblastcellline3T3anditsRSV-transformedcounterpartserveasnegativeandpositivecontrols,respectively,forantibodiestoproteinsthatarephosphorylatedasaresultoftransformationbyRSV(Roussarcomavirus).MADIn-Darbybovinekidney(MDBK)cellIysatedisplaysproteinsexpressedinbovinekidneyandPC12(ratphaeochromocytoma)cellIysatecanbeexpectedtomirrortheproteinexpressionofcellsandtissuesofneuroectodermalorigin.Likewise,JurkatcellsexpresstheproteinsuniquetohumanT-cells.Ratbrainisawidelyusedpositivecontrolforthoseproteinsthatareexclusivelyexpressedinbrain.AtrightisanexampleoftheuseofpositiveandnegativecontrolIysatesinwesternblotting.Theblotof3T3cellIysate(lane1)andRSV-transformed3T3(lane2)wasprobedwithanti-phosphotyrosineperoxidaseconjugatetorevealthoseproteinsthataretyrosine-phosphorylatedinresponsetotransformationby

Synonyms:NormalHumanFibroblastcelllysate

Product

ProductForm:Unconjugated

Formulation:ProvidedassolutioninLaemmlielectrophoresissamplebuffer.Samplesneedtobeboiledfor3-5minutes,beforeloadingthegel.

Concentration:Seevialforconcentration

ApplicationsSuggesteduse:Avolumeof10-25ulissuggestedforimmunobloting.

FunctionalAnalysis:WesternBlotting

StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles

ProductStABIlity:Reagentsarestablefortheperiodshownontheviallabelwhenstoredproperly

ShippingConditions:RoomTemperature

CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butExalphaBIOLOGicalsacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.

ProteinReference(s)

DatabaseName:UniProt

Accessionnumber:O00622

SpeciesAccession:Human

Exalpha公司简介Exalpha有限公司于2000年在马萨诸塞州雪莉（美国）成立。该公司主要通过开发和生产抗体来支持生命科学领域的研究人员和科学家。Exalpha公司产品领域Exalpha公司其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线，可提供给全球的科研实验室，制药企业，生物技术和诊断公司，医院等，目前旗下的代理公司遍布全球。Exalpha公司热销产品产品名称货号规格BrdU Cell Proliferation Assay KitX1327K1200Testschx 10 (Visual system homeobox 2) (NT) Polyclonal AntibodyX1180P250ugEBNA 3C Polyclonal AntibodyF125P250ugSURF1 Polyclonal AntibodyX1562P100ugBnip3L (IN) (nix) (bcl 2 family) Polyclonal AntibodyX1120P100ugchx 10 (Visual system homeobox 2) (CT) Polyclonal AntibodyX1179P250ugMicrophthalmia Transcription Factor (MiTF) Monoclonal AntibodyX1405M100ugEBNA 3A Polyclonal AntibodyF115P250ugCD5 PE Single-Color Reagent0054100TestsPTP mu Monoclonal AntibodyP100M100ugCD117 FITC Single-Color Reagent1172100TestsKv1.5 Potassium Channel Polyclonal AntibodyX1501P100ugN-SMase Activation Associated Factor Antigen Immunoaffinity Purified PolyclonalX2354P100ugChicken anti Glutathione S Transferase (GST), conjugated with HRPAGST-HRP0.1 mgCD7 PE Single-Color Reagent0074100TestsProtein Phosphatase 1 alpha Polyclonal AntibodyP105P100ugCD2 PE Single-Color Reagent0024100TestsKv1.6Potassium Channel Polyclonal AntibodyX1502P100ugDegenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified PolyclonalX2356P100ugCD8 PE Single-Color Reagent0084100TestsProtein Phosphatase 1 beta Polyclonal AntibodyP110P100ug