Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains), conjugated with BiotinCatalogue number: RARa/Ig/Bio

Clone	Polyclonal
Units	1 ml
Host	Rabbit

BackgroundThe reactivity of the antiserum is directed the major immunoglobulin isotypes of the rat Ig system (classes, subclasses and L-chain types), including antibodies to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

SourcePurified polyclonal IgG, including all subclasses, and pools of homogenous IgA, IgM isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

ProductBiotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Product Form: Biotin

Formulation: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

SpecificityBiotin-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG, IgA and IgM, heavy and light chains.

Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The antiSerum has not been tested for cross-reactivity in detail.

ApplicationsELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Exalpha公司简介Exalpha有限公司于2000年在马萨诸塞州雪莉（美国）成立。该公司主要通过开发和生产抗体来支持生命科学领域的研究人员和科学家。Exalpha公司产品领域Exalpha公司其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线，可提供给全球的科研实验室，制药企业，生物技术和诊断公司，医院等，目前旗下的代理公司遍布全球。Exalpha公司热销产品产品名称货号规格BrdU Cell Proliferation Assay KitX1327K1200Testschx 10 (Visual system homeobox 2) (NT) Polyclonal AntibodyX1180P250ugEBNA 3C Polyclonal AntibodyF125P250ugSURF1 Polyclonal AntibodyX1562P100ugBnip3L (IN) (nix) (bcl 2 family) Polyclonal AntibodyX1120P100ugchx 10 (Visual system homeobox 2) (CT) Polyclonal AntibodyX1179P250ugMicrophthalmia Transcription Factor (MiTF) Monoclonal AntibodyX1405M100ugEBNA 3A Polyclonal AntibodyF115P250ugCD5 PE Single-Color Reagent0054100TestsPTP mu Monoclonal AntibodyP100M100ugCD117 FITC Single-Color Reagent1172100TestsKv1.5 Potassium Channel Polyclonal AntibodyX1501P100ugN-SMase Activation Associated Factor Antigen Immunoaffinity Purified PolyclonalX2354P100ugChicken anti Glutathione S Transferase (GST), conjugated with HRPAGST-HRP0.1 mgCD7 PE Single-Color Reagent0074100TestsProtein Phosphatase 1 alpha Polyclonal AntibodyP105P100ugCD2 PE Single-Color Reagent0024100TestsKv1.6Potassium Channel Polyclonal AntibodyX1502P100ugDegenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified PolyclonalX2356P100ugCD8 PE Single-Color Reagent0084100TestsProtein Phosphatase 1 beta Polyclonal AntibodyP110P100ug