BrdU Immunohistochemistry KitCatalogue number: X1545K.1

Product Type	Kits
Units	50 Slides
Application	ImmunocytochemistryImmunohistochemistry (frozen & paraffin)

BackgroundThe BrdU Immunohistochemistry Kit is a non-isotopic enzyme immunoassay for the detection and quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S-phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. Exalpha Biologicals BrdU Immunohistochemistry Kit involves incorporation of BrdU Reagent into cells, either in tissues after injection of BrdU in experimental animals or in cells cultured in microtiter plates or on glass slides.The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture or tissue section.

ProductThe BrdU Cell Immunohistochemistry Kit contains all ingredients (antibodies, buffers and detection reagents) needed for the immunohistochemical detection and quantification of the proliferative activity of cells and tissues. The kit contains 5 control slides with BrdU prelabeled MCF-7 cells (one of which is prestained) as well as an instruction manual describing the relevant protocols for BrdU immunostaining.

ApplicationsImmunohistochemistry on formalin fixed, paraffin embedded, as well as frozen tissue sections. Furthermore this kit can be used for the immunocytochemical staining of cultured cells on glass slides or cell culture plates.Optimal concentration of the primary antibody should be evaluated by serial dilutions.

StorageUpon receipt, store entire kit at -20oC. Once the kit is thawed, you may keep it at 4ºC for 5 days. For long-term storage, it is recommended you aliquot and freeze the components at –20ºC, particularly the Streptavidin-HRP Conjugate, the Prediluted Biotinylated Sheep anti-BrdU Detector Antibody and the 4x Trypsin Concentrate.

Shipping Conditions: The Exalpha Biologicals BrdU Immunohistochemistry Kit components are shipped on blue ice.

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Kit ManualClick here to download the Kit Manual Safety Datasheet(s) for this product:EA_X1545K.1-SDS_V1/wp-content/uploads/2020/07/X1545K.1-SDS_V1.pdf

Exalpha公司简介Exalpha有限公司于2000年在马萨诸塞州雪莉（美国）成立。该公司主要通过开发和生产抗体来支持生命科学领域的研究人员和科学家。Exalpha公司产品领域Exalpha公司其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线，可提供给全球的科研实验室，制药企业，生物技术和诊断公司，医院等，目前旗下的代理公司遍布全球。Exalpha公司热销产品产品名称货号规格BrdU Cell Proliferation Assay KitX1327K1200Testschx 10 (Visual system homeobox 2) (NT) Polyclonal AntibodyX1180P250ugEBNA 3C Polyclonal AntibodyF125P250ugSURF1 Polyclonal AntibodyX1562P100ugBnip3L (IN) (nix) (bcl 2 family) Polyclonal AntibodyX1120P100ugchx 10 (Visual system homeobox 2) (CT) Polyclonal AntibodyX1179P250ugMicrophthalmia Transcription Factor (MiTF) Monoclonal AntibodyX1405M100ugEBNA 3A Polyclonal AntibodyF115P250ugCD5 PE Single-Color Reagent0054100TestsPTP mu Monoclonal AntibodyP100M100ugCD117 FITC Single-Color Reagent1172100TestsKv1.5 Potassium Channel Polyclonal AntibodyX1501P100ugN-SMase Activation Associated Factor Antigen Immunoaffinity Purified PolyclonalX2354P100ugChicken anti Glutathione S Transferase (GST), conjugated with HRPAGST-HRP0.1 mgCD7 PE Single-Color Reagent0074100TestsProtein Phosphatase 1 alpha Polyclonal AntibodyP105P100ugCD2 PE Single-Color Reagent0024100TestsKv1.6Potassium Channel Polyclonal AntibodyX1502P100ugDegenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified PolyclonalX2356P100ugCD8 PE Single-Color Reagent0084100TestsProtein Phosphatase 1 beta Polyclonal AntibodyP110P100ug