Rabbit anti Human Sphingomyelin Phosphodiesterase 3Catalogue number: X2360P

Product Type	Polyclonal Antibody
Units	100 µg
Host	Rabbit
Species reactivity	Human
Application	Western BlotWestern Blotting

BackgroundSphingomyelin phosphodiesterase 3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine.Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these two cellular events independently. It also hydrolyzes sphingosylphosphocholine. Sphingomyelin phosphodiesterase 3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It likely acts as a regulator of postnatal development and participates in bone and dentin mineralization.It is activated by unsaturated fatty acids and phosphatidylserine.it is predominantly expressed in the brain.

Synonyms: nSMase2; Neutral sphingomyelinase 2; EC 3.1.4.12

Source

Immunogen: Synthetic peptide derived from human sphingomyelin phosphodiesterase 3

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

ApplicationsAntibody can be used for Western blotting (1-5 µg/ml starting dilution).Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Predominantly expressed in the brain.

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1. Hofmann, K., et al. Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase.; Proc. Natl. Acad. Sci. U.S.A. 97:5895-5900(2000).2. Miura, Y., et al. Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases.; FEBS Lett. 557:288-292(2004).3. Marchesini, N., et al. Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells.; J. Biol. Chem. 279:25101-25111(2004).

Protein Reference(s)

Database Name: UniProt

Accession number: Q53YV1 (Human)

Species Accession: Human

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

Exalpha公司简介Exalpha有限公司于2000年在马萨诸塞州雪莉（美国）成立。该公司主要通过开发和生产抗体来支持生命科学领域的研究人员和科学家。Exalpha公司产品领域Exalpha公司其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线，可提供给全球的科研实验室，制药企业，生物技术和诊断公司，医院等，目前旗下的代理公司遍布全球。Exalpha公司热销产品产品名称货号规格BrdU Cell Proliferation Assay KitX1327K1200Testschx 10 (Visual system homeobox 2) (NT) Polyclonal AntibodyX1180P250ugEBNA 3C Polyclonal AntibodyF125P250ugSURF1 Polyclonal AntibodyX1562P100ugBnip3L (IN) (nix) (bcl 2 family) Polyclonal AntibodyX1120P100ugchx 10 (Visual system homeobox 2) (CT) Polyclonal AntibodyX1179P250ugMicrophthalmia Transcription Factor (MiTF) Monoclonal AntibodyX1405M100ugEBNA 3A Polyclonal AntibodyF115P250ugCD5 PE Single-Color Reagent0054100TestsPTP mu Monoclonal AntibodyP100M100ugCD117 FITC Single-Color Reagent1172100TestsKv1.5 Potassium Channel Polyclonal AntibodyX1501P100ugN-SMase Activation Associated Factor Antigen Immunoaffinity Purified PolyclonalX2354P100ugChicken anti Glutathione S Transferase (GST), conjugated with HRPAGST-HRP0.1 mgCD7 PE Single-Color Reagent0074100TestsProtein Phosphatase 1 alpha Polyclonal AntibodyP105P100ugCD2 PE Single-Color Reagent0024100TestsKv1.6Potassium Channel Polyclonal AntibodyX1502P100ugDegenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified PolyclonalX2356P100ugCD8 PE Single-Color Reagent0084100TestsProtein Phosphatase 1 beta Polyclonal AntibodyP110P100ug