Determining the class and subclass of a monoclonal antibody is important when selecting the best purification method. IgA and IgM are often best purified by size (gel exclusion) or affinity columns, whereas IgG_2a and IgG_2b can be purified with Protein A columns at a pH of 7-8. IgG1 monoclonals bind best to Protein A at a slightly higher pH of 8-9.

The Iso-Gold™ Rapid Mouse-Monoclonal Isotyping κ & λ Kit is a 5 minute lateral flow assay with ELISA sensitivity for determining mouse monoclonal antibody class, subclass and light chain type. The assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid. Protocols are provided for both sample types.

Specifications:

Kit Contents:

• IgG₁, IgG_2a, and IgG_2b test, 5 cassettes
• IgG3, IgA, and IgM test, 5 cassettes
• κ & λ test, 5 cassettes
• Sample Diluent Buffer, 25 mL

Storage Temperature: Store Sample Diluent Buffer refrigerated (2-8°C). Cassettes can be stored at room temperature.

Shipping Temperature: Refrigerated (2-8°C)

Stability: 2 years from date of manufacture

BioAssay Works-NakedGold®溶胶高度浓缩，可提供15 OD / mL。它们是使用不涉及传统沸腾和离心的方法生产的。它们“可结合”，因此无需浓缩即可将抗体或其他蛋白质包被在金上，从而节省了时间并减少了浪费。裸金溶胶具有出色的批间一致性。显示所有4结果默认排序默认排序按人气排序按平均评分排序按新旧程度排序按价格排序：从低到高按价格排序：从高到低价钱人气度评分显示20产品显示20产品显示40产品显示60产品裸金® 20纳米浓缩金溶胶$ 58.50 – $ 2,601.10选择选项裸金® 30纳米浓缩金溶胶$ 58.50 – $ 2,601.10选择选项裸金® 40nm的浓金溶胶- 15 OD /毫升$ 58.50 – $ 2,601.10选择选项裸金® 40nm的浓金溶胶- 50 OD / mL的$ 195.00 – $ 8,678.90