OVERVIEW:

Synonyms	MonoclonalVEGFantibody,Anti-VEGFantibody,AntiVEGFantibody,Glioma-derivedendothelialcellmitogenantibody,Folliculostellatecell-derivedgrowthfactorantibody,Folliculostellatecell-derivedgrowthfactorantibody,VPFantibody,VEGFantibody,VascularEndothelialGrowthFactorantibody,Glioma-derivedendothelialcellmitogenantibody,VPFantibody,VascularEndothelialGrowthFactorantibody
Specificity	Human
CrossReactivity	Tobedeterminedbyend-user
Applications	IHC
Immunogen	VEGFantibodywasraisedinmouseusingrecombinanthumanVEGF(E.coli)astheimmunogen

SPECIFICATIONS:

Host	Mouse
Clone	2E1
Isotype	IgG2b
Source	Ascites
MethodofPurification	VEGFantibodywasaffinitypurifiedwithproteinA
Form&Buffer	Lyophilizedproductfrom1%BSAinPBScontaining0.05%NaN3,reconstitutewith1mlofdeionisedwater

STORAGE&SAFETY:

Storage	Storeat4degCuntilreconstitution.Followingreconstitutionaliquotandfreezeat20degCforlongtermstorage

UsageAssayInformation:

UsageRecommendations	IHC:1ug/ml,WB:25ug/ml

GENERALINFORMATION:

BIOLOGicalSignificance

VascularEndothelialCellGrowthFactor(VEGF)isahomodimericproteininitiallypurifiedfrommediaconditionedbynormalbovinepituitaryfolliculostellatecellsandsecretedbyavarietyofvascularizedtissues.ItwassubsequentlyfoundtobeidenticaltoavascularpermeABIlityfactor(VPF),whichwaspreviouslyidentifiedinmediaconditionedbytumorcelllinesbaseduponitsabilitytoincreasethepermeabilityofcapillarybloodvessels.ThereportedactivitiesofVEGFincludestimulationofendothelialcellgrowth,angiogenesisandcapillarypermeability.HumanVEGFisa38.2kDahomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.VEGFisexpressedinmanyhumantumorcells,includinghumanadenocarcinoma,humanpancreaticcarcinoma,humanhepatocellularcarcinoma,renalcellcarcinoma,fibrosarcoma,HL60promyelocyticleukemia,GS9LgliomaandU937lymphomacells.Innormaltissues,VEGFexpressionhasbeenobservedinactivatedmacrophages,keratinocytes,hepatocytes,smoothmusclecellsLeydigcells,embryonicfibroblastsandbronchialandchoroidsplexusepithelium,renalglomerularvisceralepitheliumandmesangialcells.

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