Type
Recombinant
Description
EpiregulinisanEGF-relatedgrowthfactorthatbindsspecificallytoEGFR(ErbB1)andErbB4,butnotErbB2orErbB3.Itisexpressedmainlyintheplacentaandperipheralbloodleukocytes,aswellasincertaincarcinomasofthebladder,lung,kidneyandcolon.Epiregulinstimulatestheproliferationofkeratinocytes,hepatocytes,fibroblastsandvascularsmoothmusclecells.Italsoinhibitsthegrowthofseveraltumor-derivedepithelialcelllines.HumanEpiregulinisinitiallysynthesizedasaglycosylated19.0 kDatransmembraneprecursorprotein,whichisprocessedbyproteolyticcleavagetoproducea6.0 kDamaturesecretedsequence.RecombinantHumanEpiregulinisa5.6 kDamonomericprotein,containing50 aminoresidues,whichcorrespondstothematuresecretedEpiregulinsequence.
AminoAcidSequence
MVAQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFL
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineBalb/3T3cellsis≤2.0 ng/ml,correspondingtoaspecificactivityof≥5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
