Type

Recombinant

Description

EGFReceptor(EGFR,ErbB1)isatransmembraneproteinthatexertstyrosinekinaseactivityuponligand-inducedactivation.EGFRcanbeactivatedbybindingEGF,oratleastsixotherstructurallyrelatedproteinligands,includingTGFα,HB-EGF,Betacellulin(BTC),Amphiregulin,Epiregulin,andEpigen.Uponactivation,EGFRinitiatesasignalingcascade,whichincludesdimerizationandinternalization,tyrosinephosphorylation,DNAsynthesisoftargetgenesand,ultimately,cellproliferation.EGFRsignalingplaysaroleinthegrowthanddifferentiationofnormalcells,butelevatedEGFRactivityiscorrelatedwiththedevelopmentandpathogenesisofcertaincancers.RecombinantsolubleHumanEGFRisa621 aminoacidglycoproteincomprisingtheextracellulardomainofEGFR,andmigratesatanapparentMWof97.5 kDabySDS-PAGEanalysisunderreducingconditions.

AminoAcidSequence

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS

Source

ChineseHamsterOvaryCells(CHO)

Purity

95%

BIOLOGicalActivity

Testinginprogress.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Formulation

Sterilefilteredthrougha0.2 micronfilter.Lyophilizedfrom10 mMSodiumPhosphate,pH 7.5.

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C

QualityControlTest

VerifiedbyUVSpectroscopyand/orSDS-PAGEgel.

Note

ForResearchUseOnly.NotforuseinHumans!