Type
Recombinant
Description
IL-22 isamemberoftheIL-10 familyofregulatorycytokines,whichincludesIL-10,IL-19,IL-20,IL-22,IL-24 andIL-26. Membersofthisfamilysharepartialhomologyintheiraminoacidsequences,buttheyaredissimilarintheirBIOLOGicalfunctions.ProducedbyTlymphocytes,IL-22 inhibitsIL-4 productionbyTh4cells,andinducesacutephasereactantsintheliverandpancreas.IL-22 signalsthroughareceptorsystemconsistingofIL-10Rβ/CRF2–4andIL-22R,bothofwhicharemembersoftheclassIIcytokine-receptorfamily.RecombinantHumanIL-22 isa33.6 kDa,non-disulfide-linked,homodimericproteinconsistingoftwo147 aminoacidpolypeptidechains.
AminoAcidSequence
MAPISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI
Source
E.coli
Purity
98%
BiologicalActivity
Assay#1:DeterminedbyitsABIlitytoactivateSTATfollowingreceptorligandinteraction.Assay#2:MeasuredbyitsabilitytoinduceIL-10 secretioninCOLO205(humancoloncarcinomacells).TheexpectedED50 forthiseffectis0.7–1.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
