Type

Recombinant

Description

IP-10 isaCXCchemokinethatsignalsthroughtheCXCR3receptor.IP-10 selectivelychemoattractsTh1lymphocytesandmonocytes,andinhibitscytokine-stimulatedhematopoieticProgenitorcellproliferation.Additionally,itisangiostaticandmitogenicforvascularsmoothmusclecells.RecombinantHumanIP-10 isan8.7 kDaproteinconsistingof77 aminoacids,includingthefourconservedcysteineresiduespresentinCXCchemokines.

AminoAcidSequence

IPLARTVRCTCIDFHEQPLRPRAIGKLEIIPASLSCPHVEIIATMKKNNEKRCLNPESEAIKSLLKAVSQRRSKRAP

Source

E.coli

Purity

98%

BIOLOGicalActivity

Assay#1:DeterminedbyitsABIlitytochemoattractIL-2 activatedTcellsusingaconcentrationrangeof0.1–10.0 ng/ml.Assay#2:DeterminedbyitsabilitytochemoattractCXCR3-transfectedHEK293cellsusingaconcentrationrangeof100–500 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C