Type

MonoclonalAntibody

Applications

Westernblotting,Immunoprecipitation

Hosts

Mouse

Isotype

IgG1

Clone

E32

Preparation

WB(withECL):1:1000–10,000 dilution(incubateforonehouratroomtemperature)Forbestresultswithotherassays(e.g.:IP,etc),pleasedetermineoptimalworkingdilutionbytitration test.

PurificationMethod

ProteinA affinitychromatographyfrommouseascites fluid.

Formulation

1 mg/mLin10 mMPBS,pH7.2,with0.05%NaN_3.

Storage/Expiration

Storeat –20°Cforupto2 years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze-thawcycles.Storealiquotsat4°Cfor1–2 weeks.