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The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting human Interferon-gamma (IFN-γ).
The kit includes low fluorescent plates pre-coated with monoclonal capture antibody, monoclonal detection antibody, secondary reagent conjugated to fluorophore, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
A FluoroSpot reader equipped with a bandpass filter for excitation at 490 nm and emission at 510 nm (e.g., LED490, FITC-equivalent) is recommended for analysis.
Analyte Information
IFN-γInterferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.Application: |
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Reactivity: |
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Format: | FluoroSpotPLUS |
Documentation: | Datasheet/ProtocolSDS |
Contents: |
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Size: | 2 plates |
Other formats: | Click to view all Human IFN-γ products |
We have used Mabtech ELISPOT antibodies and reagents for several years. The high quality and standard and competitive price will keep us hooked for years to come. Recently we have started working with Fluorospot, which has really helped us move forward and have opened new avenues in our research. As always management and technical staff have all provided impeccable service. |