(人荧光蛋白-012/FSP-012/FSP-2)

价格
¥9520.00
货号:FSP-01A-2
浏览量:91
品牌:Mabtech
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商品描述
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ

The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting human Interferon-gamma (IFN-γ).

The kit includes low fluorescent plates pre-coated with monoclonal capture antibody, monoclonal detection antibody, secondary reagent conjugated to fluorophore, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

A FluoroSpot reader equipped with a bandpass filter for excitation at 490 nm and emission at 510 nm (e.g., LED490, FITC-equivalent) is recommended for analysis.


Analyte Information

IFN-γInterferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Ordering Information

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:Send your order toorder@mabtech.com.

Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.
Application:
  • FluoroSpot
Reactivity:
  • Human
Format:FluoroSpotPLUS
Documentation:Datasheet/ProtocolSDS
Contents:
  • BAM-conjugated detection mAb (7-B6-1)
  • Anti-BAM-490
  • anti-CD3 mAb
  • Anti-CD28 mAb (CD28-A)
  • Fluorescence enhancer
  • 2 Pre-coated FluoroSpot plates (mAb 1-D1K)
Size:2 plates
We have used Mabtech ELISPOT antibodies and reagents for several years. The high quality and standard and competitive price will keep us hooked for years to come. Recently we have started working with Fluorospot, which has really helped us move forward and have opened new avenues in our research. As always management and technical staff have all provided impeccable service.
Mabtech公司成立于1986年,最初源自瑞典斯德哥尔摩大学免疫学系,目前已成为ELISpot和T细胞检测技术的全球领先者。细胞应答分析技术之一,是探讨感染,癌症,过敏和自身免疫性疾病等众多转变中T细胞功能的最理想的研究手段。因其具有结果直观和可以通过高通量检测等优点,业已成为新疫苗实验研究和临床评价的标准方法。作为ELISpot产品的补充,Mabtech公司很快推出了一系列即用型细胞因子ELISA试剂盒,可以方便,准确地检测血清和血浆等多种样品中的细胞因子水平。MABTECH公司以卓尔不群的创新能力和稳定可靠的产品质量,不断开发出适应前沿科学研究和临床大规模筛选需求的优质产品.Mabtech公司依靠一支精干和敬业的团队,为用户提供最好的产品和最佳的技术服务。除位于斯德哥尔摩的公司总部和研究实验室,Mabtech公司在瑞典,法国,德国和美国均设有办事处,与全球高端科研机构保持紧密的合作并为全球用户提供快捷的物流。Mabtech公司提供多种高品质的ELISA和ELISpot试剂盒,用于检测多种种属细胞因子和相关的免疫效应分子。Mabtech公司的抗体也可单独包装,供用户进行流式细胞术和免疫细胞化学等实验研究。MABTECH AB是一家私营生物技术公司成立于1986年,从免疫学的斯德哥尔摩大学系新兴,MABTECH已成为酶联免疫斑点和T细胞测量的全球领导者。ELISpot代表了分析特定T细胞反应的最灵敏和最简便的方法之一,是研究T细胞在例如感染,癌症,变态反应和自身免疫性疾病中的作用时的理想选择。凭借其直接的性能和筛选大量样品的可能性,ELISpot已成为新疫苗研究和临床评估的标准方法。为了补充ELISpot产品,我们最近推出了一系列完整的即用型ELISA试剂盒,可轻松测量包括血清和血浆在内的各种样品中的细胞因子水平。通过将创新发展与高质量相结合,我们的目标是继续提供能够满足一线研究人员以及临床研究人员的需求的产品,这些产品在标准化条件下需要进行大规模筛选。我们拥有一支高素质和敬业的员工队伍,致力于始终提供最优质的产品和最佳的技术支持。总部和研究实验室位于斯德哥尔摩,但为了与研究界紧密合作并能够在全球范围内快速交付产品,我们还在澳大利亚,法国,德国和美国设有当地办事处。在其他几个国家/地区,我们通过选定的分销商开展工作。